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Tripidium arundinaceum

ABSTRACT: Drought transcriptome sequencing of leaves of Erianthus arundinaceus and Saccharum hybrid

PROVIDER: PRJNA770031 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19571075_1.fastq.gz	Fastqsanger.gz
	SRR19571075_2.fastq.gz	Fastqsanger.gz
	SRR19571076_1.fastq.gz	Fastqsanger.gz
	SRR19571076_2.fastq.gz	Fastqsanger.gz
	SRR19571077_1.fastq.gz	Fastqsanger.gz

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Tripidium arundinaceum cultivar:EA001

Project description:Erianthus arundinaceus EA001 Resequencing

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Tripidium arundinaceum cultivar:IK 76-48

Project description:Erianthus arundinaceus IK 76-48 Resequencing

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Project description:Drought transcriptome sequencing of roots of Erianthus arundinaceus and Saccharum hybrid

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Tripidium arundinaceum isolate:BC5 progeny of a cross between Saccharum spp. and Erianthus arundinaceus | cultivar:1679-33

Project description:Tripidium arundinaceum isolate:BC5 progeny of a cross between Saccharum spp. and Erianthus arundinaceus | cultivar:1679-33 Genome sequencing

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Tripidium arundinaceum

Project description:Tripidium arundinaceum Genome sequencing and assembly

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Chromosomal Characterization of <i>Tripidium arundinaceum</i> Revealed by Oligo-FISH.

Project description:Sugarcane is of important economic value for producing sugar and bioethanol. Tripidium arundinaceum (old name: Erianthus arundinaceum) is an intergeneric wild species of sugarcane that has desirable resistance traits for improving sugarcane varieties. However, the scarcity of chromosome markers has hindered the cytogenetic study of T. arundinaceum. Here we applied maize chromosome painting probes (MCPs) to identify chromosomes in sorghum and T. arundinaceum using a repeated fluorescence in situ hybridization (FISH) system. Sequential FISH revealed that these MCPs can be used as reliable chromosome markers for T. arundinaceum, even though T. arundinaceum has diverged from maize over 18 MYs (million years). Using these MCPs, we identified T. arundinaceum chromosomes based on their sequence similarity compared to sorghum and labeled them 1 through 10. Then, the karyotype of T. arundinaceum was established by multiple oligo-FISH. Furthermore, FISH results revealed that 5S rDNA and 35S rDNA are localized on chromosomes 5 and 6, respectively, in T. arundinaceum. Altogether, these results represent an essential step for further cytogenetic research of T. arundinaceum in sugarcane breeding.

| S-EPMC8395171 | biostudies-literature

Repetitive Sequence Barcode Probe for Karyotype Analysis in <i>Tripidium arundinaceum</i>.

Project description:The barcode probe is a convenient and efficient tool for molecular cytogenetics. Tripidium arundinaceum, as a polyploid wild allied genus of Saccharum, is a useful genetic resource that confers biotic and abiotic stress resistance for sugarcane breeding. Unfortunately, the basic cytogenetic information is still unclear due to the complex genome. We constructed the Cot-20 library for screening moderately and highly repetitive sequences from T. arundinaceum, and the chromosomal distribution of these repetitive sequences was explored. We used the barcode of repetitive sequence probes to distinguish the ten chromosome types of T. arundinaceum by fluorescence in situ hybridization (FISH) with Ea-0907, Ea-0098, and 45S rDNA. Furthermore, the distinction among homology chromosomes based on repetitive sequences was constructed in T. arundinaceum by the repeated FISH using the barcode probes including Ea-0663, Ea-0267, EaCent, 5S rDNA, Ea-0265, Ea-0070, and 45S rDNA. We combined these probes to distinguish 37 different chromosome types, suggesting that the repetitive sequences may have different distributions on homologous chromosomes of T. arundinaceum. In summary, this method provide a basis for the development of similar applications for cytogenetic analysis in other species.

| S-EPMC9224303 | biostudies-literature

Tripidium arundinaceum

Project description:Tripidium arundinaceum overview

| PRJNA980860 | ENA

Tripidium arundinaceum

Project description:Tripidium arundinaceum Raw sequence reads

| PRJNA1086181 | ENA

Vast differences in organ-specific rhythms of transcription in field-grown sugarcane

Project description:Sugarcane (Saccharum hybrid, SP80-3280) was grown in the field in Araras (Brazil) for 9 months. Leaves +1 (F1), internodes 1&2 (I1), and internodes 5 (I5) were harvested every 2 h for 26 h, starting 2h before dawn.

2019-04-10 | GSE129543 | GEO

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