Project description:The sRNA F6 in M.tb is under the control of SigF, a non essential sigma factor known to be induced during nutrient starvation. We knocked out this sRNA in M. tb and subjected the strain to 24 hours starvation. RNA/RNA microarrays were then used in order to look at effects in gene expression between wildtype H37Rv and F6 knockout.
Project description:Working with primary human myogenic progenitors (hMPs), we developed a high-content screen to discover molecules that enhanced myogenic function and identified NAM and PN as modulators of myogenic proliferation and differentiation. We characterized genome-wide expression profiles of NAM-, PN-treated and NAM/PN-treated hMPs with the goal to uncover the mechanisms of action of NAM and PN and the potential additivity from the combination at the molecular level in hMPs.