Project description:Epitranscriptomic regulation controls information flow through the central dogma and provides new opportunities to control the cellular outputs at the RNA level. However, both basic science and translational applications are limited by a lack of methods to target RNA specifically. Here we present a novel protein engineering approach to construct probgrammable RNA regulatory systems from human parts: CRISPR/Cas-Inspired RNA Targeting System (CIRTS).
Project description:Comprehensive RNA-seq experiments to measure the expression of homoeologs across different tissues, as a part of the Xenopus laevis genome project. This work is funded by Agency Japanese Ministry of Education, Culture, Sports, Science and Technology (MEXT; "Genome Science" Grant ID 221S0002).
Project description:Comprehensive RNA-seq experiments to measure the expression of homoeologs across different developmental stages, as a part of the Xenopus laevis genome project. This work is funded by Agency Japanese Ministry of Education, Culture, Sports, Science and Technology (MEXT; "Genome Science" Grant ID 221S0002).
2016-10-24 | GSE73430 | GEO
Project description:Regional Science Foundation project of National Natural Science Foundation of China (31960381)
Project description:We previous identified enhancers that regulate MYC expression in K562 cells (Fulco et al. Science 2016). Here, we perturbed MYC enhancers with individual CRISPRi gRNAs and performed ChIP-seq to study the effects on chromatin state.
2023-11-14 | GSE225157 | GEO
Project description:Chongqing Science and Technology Bureau
Project description:Using iChIP, we map H3K27Ac, H3K4me3 and H3K4me1 in small populations (HSPCs) in the presence or absence of Med12 and identify affected super-enhancers. iChIP was performed using previously published protocols (Lara-Astiaso et al, 2014 Immunogenetics. Chromatin state dynamics during blood formation - Science, 345, 6199)