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Microbotryum violaceum

ABSTRACT: Whole genome sequencing of a1 and a2 haploid cells of Microbotryum violaceum

PROVIDER: PRJNA771266 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR17778145_subreads.fastq.gz	Fastqsanger.gz
	SRR17778146_subreads.fastq.gz	Fastqsanger.gz
	SRR17778147_subreads.fastq.gz	Fastqsanger.gz
	SRR17778148_subreads.fastq.gz	Fastqsanger.gz

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Project description:Antibiotic resistance can arise by several mechanisms, including mutation in transcription factors that regulate drug efflux pumps. In this work, we identified EmrR as a MarR family transcription factor involved in antibiotic resistance in Chromobacterium violaceum, a Gram-negative bacterium that occurs in soil and water and can act as a human opportunistic pathogen. Antibiogram and minimum inhibitory concentration (MIC) assays showed that the ΔemrR mutant presented increased resistance to the antibiotic nalidixic acid in respect to the wild-type strain. The emrR gene is near to a putative operon emrCAB, which encode the efflux pump EmrCAB. DNA Microarray analysis showed that EmrR represses the emrCAB operon and some other putative transporters. Northern blot assays validated that EmrR represses the emrCAB operon and this repression can be released by salicylate, but not other compounds such as nalidixic acid or ethidium bromide. Electrophoretic mobility shift assays (EMSA) showed that EmrR binds directly to the promoter regions of emrR, emrCAB and other genes to exert negative regulation. Therefore, in response to compounds as salicylate, EmrR derepresses the operon emrCAB causing overexpression of the efflux pump EmrCAB and increased resistance to nalidixic acid in C. violaceum.

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