ABSTRACT: Genomic based transmission analysis of carbapenem-resistant Pseudomonas aeruginosa at a tertiary care centre in Cologne (Germany) from 2015 to 2020
Project description:Transmission analysis of carbapenem-resistant Pseudomonas aeruginosa at a tertiary care centre in Cologne (Germany) from 2015 to 2020
Project description:This experiment aims at analyzing genotype profiles of wild-type and apomictic BRS CIRAD-302 rice. Leaf samples from plants of the parental, F1, F2, T0, T1, and T2 were used for DNA purification and library preparation for Illumina sequencing (2x150 bp), performed by the Max Planck-Genome-centre Cologne, Germany (https://mpgc.mpipz.mpg.de/home/).
Project description:This experiment aims at analyzing crossover distribution in male and female meiosis, in the Arabidopsis. Wild-type Col plant was crossed with Ler plant to produce F1 hybrid. Then, the F1 hybrid was crossed as female or as male with wild-type Col to generate two BC1 populations. Leaf samples from plants of the obtained BC1 populations were used for DNA purification and library preparation for Illumina sequencing (HiSeq 3000 2x150 bp), performed by the Max Planck-Genome-centre Cologne, Germany (https://mpgc.mpipz.mpg.de/home/) and Novogene.
Project description:This experiment aims at analyzing crossover distribution in male and female meiosis, in the Arabidopsis pure Ler. Wild-type Ler seeds were subjected to EMS mutagenesis, and four independent M2 plants were crossed to produce two independent F1*, which were heterozygous for a set of EMS mutations. Then, the two F1* plants were reciprocally crossed to generate two F1 populations. Leaf samples from plants from the obtained F1 populations were used for DNA purification and library preparation for Illumina sequencing (HiSeq 3000 2x150 bp), performed by the Max Planck-Genome-centre Cologne, Germany (https://mpgc.mpipz.mpg.de/home/).
Project description:This experiment aims at analyzing crossover distribution in female and male meiosis, in the Arabidopsis pure Col. Wild-type Col seeds were subjected to EMS mutagenesis, and four independent M2 plants were crossed to produce two independent F1*, which were heterozygous for a set of EMS mutations. Then, the two F1* plants were reciprocally crossed to generate two F1 populations. Leaf samples from plants from the obtained F1 populations were used for DNA purification and library preparation for Illumina sequencing (HiSeq 3000 2x150 bp), performed by the Max Planck-Genome-centre Cologne, Germany (https://mpgc.mpipz.mpg.de/home/).
Project description:This experiment aims at analyzing crossover distribution in male and female meiosis, in the Arabidopsis pure Col. Wild-type Col seeds were subjected to EMS mutagenesis, and four independent M2 plants were crossed to produce two independent F1*, which were heterozygous for a set of EMS mutations. Then, the two F1* plants were reciprocally crossed to generate two F1 populations. Leaf samples from plants from the obtained F1 populations were used for DNA purification and library preparation for Illumina sequencing (HiSeq 3000 2x150 bp), performed by the Max Planck-Genome-centre Cologne, Germany (https://mpgc.mpipz.mpg.de/home/).
Project description:This experiment aims at analyzing crossover distribution in male and female meiosis, in the Arabidopsis pure Ler. Wild-type Ler seeds were subjected to EMS mutagenesis, and four independent M2 plants were crossed to produce two independent F1*, which were heterozygous for a set of EMS mutations. Then, the two F1* plants were reciprocally crossed to generate two F1 populations. Leaf samples from plants from the obtained F1 populations were used for DNA purification and library preparation for Illumina sequencing (HiSeq 3000 2x150 bp), performed by the Max Planck-Genome-centre Cologne, Germany (https://mpgc.mpipz.mpg.de/home/).
Project description:This experiment aims at analyzing crossover distribution in male and female meiosis, in the Arabidopsis zyp1 mutant compared to wild type. Plants heterozygous for the zyp1-1 mutation (Col) were crossed as female with plants heterozygous for the zyp1-6 mutation (Ler). Wild type and zyp1-1/zyp-6 plants were selected among the F1s, and crossed as male or as female with wild-type Col. Leaf samples from plants from the four obtained BC1 populations were used for DNA purification and library preparation for Illumina sequencing (HiSeq 3000 2x150 bp), performed by the Max Planck-Genome-centre Cologne, Germany (https://mpgc.mpipz.mpg.de/home/).