Project description:Gene expression analysis of four different treatments of Aspergillus nidulans. reference line (A.nidulans), line A (A.nidulans + Streptomyces rapamycinicus), line B (A.nidulans + orsellinic acid), line C (A.nidulans + lecanoric acid)
Project description:Gene expression analysis of four different treatments of Aspergillus nidulans. reference line (A.nidulans), line A (A.nidulans + Streptomyces rapamycinicus), line B (A.nidulans + orsellinic acid), line C (A.nidulans + lecanoric acid) For each of these treatments 2 biological replicates were made.
Project description:Aspergillus nidulans competes in its natural environment with other microorganisms. It has been known that the bacterium Streptomyces rapamycinicus induces the production of Orsellinic acid in A. nidulans and we found that the major trigger of this induction is Polaramycin B produced by S. rapamycinicus. Here we show the transcriptome of A. nidulans following treatment with 0.5µg/ml Polaramycin B after 30 minutes and 3 hours.
Project description:Mining of fungal genomes uncovered their great potential for the production of novel secondary metabolites (SMs). However most of them stay silent under standard laboratory cultivation conditions. Co-cultivation of fungi with organism that occur in their natural habitat has shown to be trigger for the activation of such silent SM gene clusters. Recently, we showed that the cultivation of Aspergillus nidulans with the bacterium Streptomyces rapamycinicus leads to the activation of the orsellinic acid gene cluster. Hence we decided to study this interaction further to gain insight into the regulation of SM gene clusters and more specifically to study the chromatin remodelling network actuve upon co-cultivation of the two organisms. This study gives novel insight into the regulation of the orsellinic acid gene cluster and the interaction of the two organisms. To the best of our knowledge this is the first report of mapping the chromatin landscape of microbial interactions, making this study a role model for the analysis of similar systems.
Project description:We identified genome-wide binding regions of NdgR in Streptomyces coelicolor using chromatin immunoprecipitation sequencing (ChIP-seq). We constructed 6×myc-tagged NdgR strain using homologous recombination with myc-tagging vector. Analysis of the sequencing data aligned to Streptomyces coelicolor genome database (NC_003888).
