Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

CEBPB-dependent transcriptome in NRF2-activated non-small cell lung cancer cell line A549 [RNA-seq]

ABSTRACT: CEBPB-dependent transcriptome in NRF2-activated non-small cell lung cancer cell line A549 [RNA-seq]

PROVIDER: PRJNA772793 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

CEBPB-dependent enhancer formation in NRF2-activated non-small cell lung cancer cell line A549 [ChIP-seq]

Project description:CEBPB-dependent enhancer formation in NRF2-activated non-small cell lung cancer cell line A549 [ChIP-seq]

| PRJNA772792 | ENA

CEBPB-dependent transcriptome in NRF2-activated non-small cell lung cancer cell line A549 [RNA-seq]

Project description:CEBPB was knocked down in A549 cells, and transcriptomic alterations were examined.

2022-10-08 | GSE186187 | GEO

CEBPB-dependent enhancer formation in NRF2-activated non-small cell lung cancer cell line A549 [ChIP-seq]

Project description:CEBPB was knocked down in A549 cells, and H3K27ac deposition were examined.

2022-10-08 | GSE186186 | GEO

Comparison of gene expression profiles of A549 cell treated with NRF2 siRNA and control siRNA

Project description:To examine the role of NRF2 in accelerating cell proliferation and to identify the target genes responsible for this function, transcriptome analysis was performed using A549 cells, in which NRF2 is constitutively activated. NRF2 was knocked down by siRNA against NRF2, and the gene expression profile was compared with that of A549 cells treated with control siRNA. To exclude off-target effects, three different siRNAs against NRF2 was independently applied. NRF2 siRNA or control siRNA was transfected into A549 cells. Cells were harvested 24 hours after transfection, and total RNA was purified.

2011-04-01 | E-GEOD-28230 | biostudies-arrayexpress

Comparison of gene expression profiles of A549 cell treated with NRF2 siRNA and control siRNA

2011-04-01 | GSE28230 | GEO

Identification of Nrf2-regulated genes in A549 lung cancer cells by oligonucleotide microarray

Project description:To elucidate the mechanisms by which Nrf2 regulates cell growth, we performed global gene expression profiling of A549 lung cancer cells with knockdown of Nrf2. Gene networks associated with carbohydrate metabolism and drug metabolism were significantly downregulated in Nrf2-depleted A549 cells. Gene Set Enrichment Analysis revealed significant enrichment of genes associated with carbohydrate catabolic processes, positive regulation of metabolic processes, PPP, and arachidonic acid metabolism. In summary, this analysis revealed that Nrf2 positively regulates transcription of genes that play key roles in central carbon metabolism. A549 cells were transfected with non targeting NS siRNA or siRNA targeting Nrf2. Mock transfected A549 cells were treated with transfection reagent alone. We had 3 biological replicates for each of the 3 groups. Ninty six hours post transfection, cells were lysed and total RNA was isolated.

2013-07-04 | E-GEOD-38332 | biostudies-arrayexpress

Expression data from KEAP1 overexpression and NRF2 siRNA knockdown of A549 NSCLC cell

Project description:KEAP1 overexpressed and NRF2 siRNA knockdown A549 NSCLC cells were used to identify downstream genes of NRF2 pathway separately and by combinatorial analysis. We used triplicate microarrays of transfected A549 cells with mKeap1-GFP for overexpression, siRNAs targeting NRF2 for knockdown and siGFP as control respectively. As a result, we identified several genes which are involved in cancer metabolic functions in these cells. We used microarrays to identify the gene downregulated in both KEAP1 overexpressed and NRF2 siRNA knockdown A549 NSCLC cells and found a subset of downregulated genes which are involved in metabolic functions.

2017-08-02 | GSE94393 | GEO

Nrf2 regulated genes in A549 cells

Project description:Nrf2 regulated genes in A549 cells

| PRJNA451429 | ENA