Project description:Salmo trutta caspius population is an important species for aquaculture and livestock industry. Moreover, these species are used for studies on molecular markers. Mitochondrial genomic is also beneficial for phylogenetic studies in salmonid species. They are applied for maternal traits whereas paternal traits are related to nuclear genomics. Mtgenomics will be genetically highly divergent indicating that they may represent distinct and potentially locally adapted gene pools. Evolutionary history of the salmo taxa such as brown trout, salmo salar, and s. trutta populations has been studied. In the present study, 50 samples of the Iranian S.t. caspius population were collected from three regions [Tonekabon (Cheshmekileh Roud), Ramsar (Safa Roud), and Talesh (Nav roud)]. The PCR product was carried out for machinery sequencing. Mtgenomic in S.t. caspius was deposited in GenBank under accession no. LC011387.1. Evolutionary analyses were conducted using MEGA7 software. The analysis involved 50 nucleotide sequences. A close relationship was observed between the samples.
Project description:Salmo caspius Kessler, 1877 is one of the most commercially important species of Salmonidae in the southern basin of the Caspian Sea. The occurrence of its wild populations has undergone sever reduction during the last years. In spite of the yearly restocking activity, still no progress on the recovery of its wild population has been observed. Hence, the present study was done in order to assess the efficiency of the current restocking activity in the southern Caspian basin in term of genetic diversity. DNA extracts of 32 S. caspius from four different groups were screened using 62621 genome-wide single nucleotide polymorphisms (SNP). The overal genetic diversity and Fst values were 0.18 and 0.08, respectively. Considering the observed admixture pattern and the positive values for inbreeding coeficient it seems that S. caspius suffers from its small effective population size. In order to obtain the maximum performance, alonside with expanding the size of brood stocks, rehabilitation of the habitats and spawning rivers of this nationally endangered species is of great importance.