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Differential gene expressionin cMet-enriched T cell populations

ABSTRACT: Differential gene expressionin cMet-enriched T cell populations

PROVIDER: PRJNA773097 | ENA |

REPOSITORIES: ENA

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Differential gene expressionin cMet-enriched T cell populations

Project description:Control (cMet+ T cells less than 0.5%) and cMet-enriched (7%) T cells were activated with plate-bound anti-CD3/CD28 and gene tracription was measured

2022-09-15 | GSE186270 | GEO

Sequencing of microRNA in spermatogonial stem cell enriched cell populations.

Project description:MicroRNAs (miRs) play a key role in the control of gene expression in a wide array of tissue systems where their functions include the regulation of self-renewal, cellular differentiation, proliferation, and apoptosis. However, the functional importance of individual miRs in controlling spermatogonial stem cell (SSC) homeostasis has not been investigated. Using high-throughout sequencing, we profiled the expression of miRs in the Thy1+ testis cell population, which is highly enriched for SSCs, and the Thy1- cell population, composed primarily of testis somatic cells. In addition, we profiled the global expression of miRs in cultured germ cells, also enriched for SSCs. Our results demonstrate that miR-21, along with miR-34c, -182, -183, -146a, -465a-3p, -465b-3p, -465c-3p, and -465c-5p are preferentially expressed in the Thy1+ SSC-enriched population, as compared to Thy1- somatic cells, and we further observed that Thy1+ SSC-enriched testis cells and SSC-enriched cultured germ cells share remarkably similar miR expression profiles. Spermatogonial Stem Cell enriched cell populations (freshly isolated and short-term cultured) and somatic cell populations were isolated from C57B/L6 mouse donors and subjected to small RNA isolation and sequencing.

2011-07-20 | E-GEOD-29613 | biostudies-arrayexpress

miRNA expression profiling of enriched male germ cell populations

Project description:MicroRNA is essential for the process of spermatogonesis, however analysis of its change in expression within germ cells during this process has been limited. We set out to examine the change in the miRNA expression profile of highly enriched mouse germ cell populations in vairous developmental stages.These inlcuded postnatal gonocytes, day 7-9 spermatogonia as well as pachytene spermatocytes and round spermatids from adult mice Gonocytes (day1) and spermatogonia (day 7-9), pachytene spermatocytes and round spermadits (adult) were enrichged by 2-4% BSA gradient sedementation. Three biological replicates of each cell type were included.

2012-03-17 | E-GEOD-36566 | biostudies-arrayexpress

Gene expression of Cell populations

Project description:Gene expression of Cell populations

| PRJNA528074 | ENA

miRNA expression profiling of enriched male germ cell populations

2012-03-17 | GSE36566 | GEO

Single cell RNA sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis

Project description:Single cell RNA sequencing reveals differential cell cycle activity in key cell populations during nephrogenesis

| PRJNA664190 | ENA

Microenvironment Cell Populations

Project description:Microenvironment Cell Populations

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A novel FACS-based method to isolate enriched populations of neurons, astrocytes, and microglia

Project description:We developed a novel FACS-based method to isolate enriched populations of neurons, astrocytes, and microglia. Whole mouse brains were digested, and populations of neurons, astrocytes, and microglia were subsequently enriched by FACS using a combination of positive gating on cell-type specific markers and stringent negative selection against markers of oligodendrocytes and endothelial cells. We confirmed a strong enrichment of corresponding cell-type specific genes in isolated populations and a reduction in gene markers of endothelial cells and oligodendrocytes, demonstrating that highly pure populations of neurons, astrocytes, and microglia were obtained.

2020-09-16 | GSE139000 | GEO

The genomic distribution and gene expression profiling of cardiomyocyte-enriched populations

Project description:The genomic distribution and gene expression profiling of cardiomyocyte-enriched populations

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Transformation of enriched mammary cell populations with Polyoma Middle T Antigen influences tumor subtype and metastatic potential

Project description:Breast cancer is a multifaceted disease, exhibiting significant molecular, histological, and pathological diversity. Factors that impact this heterogeneity are poorly understood; however, transformation of distinct normal cell populations of the breast may generate different tumor phenotypes. Our previous study demonstrates that the polyomavirus middle T antigen (PyMT) oncogene can establish diverse tumor subtypes when broadly expressed within mouse mammary epithelial cells. Herein, we assess the molecular, histological, and metastatic outcomes from distinct mammary cell populations transformed with PyMT. By combining several methodologies, including lentiviral infection, cell sorting, and transplantation, we have characterized tumors arising from enriched populations of mammary epithelial cells. We have found that expression of PyMT within different cell populations influences tumor histology, molecular subtype, and metastatic potential.

2015-09-08 | GSE64453 | GEO

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