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Development of molecular indicators for adverse health responses of effluent exposure in caged longnose dace (Rhinichthys cataractae) in ACWA raceways

ABSTRACT: Development of molecular indicators for adverse health responses of effluent exposure in caged longnose dace (Rhinichthys cataractae) in ACWA raceways

PROVIDER: PRJNA773417 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16522800_1.fastq.gz	Fastqsanger.gz
	SRR16522800_2.fastq.gz	Fastqsanger.gz
	SRR16522801_1.fastq.gz	Fastqsanger.gz
	SRR16522801_2.fastq.gz	Fastqsanger.gz
	SRR16522802_1.fastq.gz	Fastqsanger.gz

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Development of molecular indicators for adverse health responses of effluent exposure in caged longnose dace (Rhinichthys cataractae) in ACWA raceways

Project description:The goal of this experiment was to assess transcriptomic responses in caged longnose dace (Rhinichthys cataractae) (LND) females and males exposed to 5% final effluent over time to identify potential transcriptomic markers that can be used as reliable indicators and that can further be explored in field-based ecotoxicogenomic studies in the Bow River. We used the Advancing Canadian Wastewater Assets (ACWA) artificial streams to cage LND for 28 days exposed to either 5% final effluent or control.

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Project description:The Patancheru area near Hyderabad in India is recognized as a key link in the global supply chain for many bulk drugs. A central treatment plant receives wastewater from about 90 different manufacturers and the resulting complex effluent has contaminated surface, ground and drinking water in the region. Ecotoxicological testing of the effluent has shown adverse effects for several organisms, including aquatic vertebrates. In an attempt to start investigating how exposure to effluent-contaminated water may affect humans and other terrestrial vertebrates, rats were tube-fed effluent. Results from microarray and quantitative polymerase chain reaction assays indicated, however, no marked effects on hepatic gene expression after five days exposure. Neither did a clinical analysis of blood serum constituents used as biomarkers for human disease reveal any significant changes, nor were there any effects on weight gain. Taken together, we could not find evidence for any acute toxicity in the rat; however, we cannot rule out that higher doses of effluent or a longer exposure time may still be associated with risks for terrestrial vertebrates.

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