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Real-time quantitative PCR analysis of human EMT genes in MCF-7 cells [Data S8]

ABSTRACT: Real-time quantitative PCR analysis of human EMT genes in MCF-7 cells [Data S8]

PROVIDER: PRJNA773510 | ENA |

REPOSITORIES: ENA

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Real-time quantitative PCR analysis of human EMT genes in MCF-7 cells [Data S8]

Project description:Human EMT PCR array PCR array data for expression of EMT genes in MCF-7 cells after treating with ND and T2D adipocytes EVs.

2021-10-22 | GSE186366 | GEO

Real-time quantitative PCR analysis of human EMT genes in MCF-7 cells [Data S1]

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Real-time quantitative PCR analysis of MCF-7 cells after treating with TSP5-overexpressing cells' exosomes [Data S5]

Project description:Human EMT PCR array Real-time quantitative PCR analysis of human EMT genes in PCR array data for expression of EMT genes in MCF-7 cells, upon treatment with exosomes released from TSP5-overexpressing human primary adipocytes (ND; ABM-007), compared to exosomes released from matched control adipocytes transduced with control lentiviral vector [Data S5] PCR array data for expression of EMT genes in MCF-7 cells after treating with TSP5 overexpressing cells' exosomes

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Real-time quantitative PCR analysis of human EMT genes in MCF-7 cells [Data S9]

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Real-time quantitative PCR analysis of human EMT genes in MCF-7 cells [Data S1]

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Project description:Real-time quantitative PCR analysis of human EMT genes in MCF-7 cells [Data S9]

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Comparison of LC-MS/MS and real-time PCR assays for the efficient detection of potentially allergenic silkworm

Project description:The silkworm (Bombyx mori) has long been considered a source of food and medicine due to its high nutritional, medicinal, and economic value in East Asia. However, in some sensitive individuals, silkworm consumption can cause allergenic reactions such as vomiting, asthma, and anaphylaxis. Therefore, the development of a reliable method for silkworm detection is required to avoid such allergenic incidents. In this study, two different methods (liquid chromatography combined with mass spectrometry [LC-MS/MS] and real-time polymerase chain reaction [PCR]) were developed to determine an efficient technique for silkworm detection in foods. The developed methods demonstrated high sensitivity in detecting the silkworm in processed foods. Silkworm-spiked model cookies were used to confirm the sensitivity of both LC-MS/MS (0.0005%) and real-time PCR (0.001%). These methods were found to be useful for detecting the silkworm in foods and avoiding allergenic reactions. To the best of our knowledge, this is the first study to compare LC-MS/MS and real-time PCR for silkworm detection in complex processed foods.

2024-05-24 | PXD042494 | Pride

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