Project description:Expression of bumblebees (Bombus terrestris) from four colonies exposed to 3 different genotypes of the trypanosome parasite Crithidia bombi
Project description:There are multiple types of small RNAs that may affect rice pollen’s development. To investigate the small RNA populations’ change during rice pollen development, 13-40 nt RNA were extracted from uninucleate microspores (UNM) and bicellular pollen (BCP) for high throughput sequencing. Together with our laboratory’s previous published rice tricellular pollen (TCP) small RNA sequencing data (GSM722128), sharp increase of tRNA fragments (tRFs) in BCP stage and a slightly decreased tRFs in TCP were found. Among which, new lengths of tRFs were also discovered. Our work accomplished the knowledge about tRFs in rice pollen development.
Project description:Self-inhibition of pollen tubes plays a key role in SI, but the underlying mechanism in Camellia oleifera is poorly understood. Collection of secreted proteins from Camellia oleifera pollen tubes and ovaries for high-throughput sequencing.
Project description:Transcriptional analysis of stigmas was performed to identify molecules functioning in compatible pollination. Stigmas of A. thaliana Col-0 were collected (i) at 0 min or 15 min after pollination and (ii) before or at 15 min after pollen coat adhesion, and used for microarray analysis. Genes up-regulated after pollination and after pollen coat adhesion were identified. .
