Project description:Insect control programs utilizing the sterile insect technique (SIT) are more efficient when all insects released are male. One approach to producing all males in a mass-rearing setting is development of tetracycline-repressible (Tet-Off) transgenic sexing systems. Tetracycline can have adverse impacts on insect health, therefore we reared screwworm, Cochliomyia hominivorax, like Tet-Off strains to investigate the impact on fitness, the midgut microbiota, and midgut transcriptomes. Overall, the impacts on the microbiota and transcriptome did not have adverse effects on the strain fitness, and changes caused by tetracycline exposure were not inherited by the offspring mimicking a male-only generation.
2025-03-06 | GSE290117 | GEO
Project description:Microbial diversity in sterile medfly guts after probiotic treatment
Project description:PCR read-off microarrays: The ability to efficiently and economically generate libraries of defined pieces of DNA would have a myriad of applications, not least in the area of defined or directed sequencing and synthetic biology, such as protein engineering and aptamer library screening, but also in applications associated with encoding and tagging. By this approach DNA microarrays were used to allow the linear amplification off immobilized DNA sequences on an array followed by PCR amplification. Arrays of increasing sophistication (1; 10; 3,875; 10,000 defined sequences) were used to validate the process, with sequences verified by selective hybridization to a complementary DNA microarray and DNA sequencing. This technique offers an economical and efficient way of producing hundreds to thousands of specific DNA libraries, while the DNA-arrays can be used as factories allowing specific DNA oligonucleotide pools to be generated with or without masking.
Project description:This analysis compares gene expression between 1 day old and 4 day old virgin medfly, Ceratitis capitata females This analysis compares gene expression between 1 day old and 4 day old virgin medfly, Ceratitis capitata females. Samples where made from the heads of the females.
Project description:This analysis compares gene expression between 1 day old and 4 day old virgin medfly, Ceratitis capitata, males This analysis compares gene expression between 1 day old and 4 day old virgin medfly, Ceratitis capitata males. Samples where made from the heads of the males.
Project description:This analysis compares gene expression between 4 day old mated and 4 day old virgin medfly, Ceratitis capitata females This analysis compares gene expression between 4 day old mated and 4 day old virgin medfly, Ceratitis capitata females. Samples where made from the heads of the females.
Project description:This analysis compares gene expression between 4 day old mated and 4 day old virgin medfly, Ceratitis capitata, males This analysis compares gene expression between 4 day old mated and 4 day old virgin medfly, Ceratitis capitata, males. Samples where made from the heads of the males.
