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Next-generation sequencing facilitates quantitative analysis of rat BMSCs in fast, medium or slow stress relaxation SA hydrogel

ABSTRACT: Next-generation sequencing facilitates quantitative analysis of rat BMSCs in fast, medium or slow stress relaxation SA hydrogel

PROVIDER: PRJNA775649 | ENA |

REPOSITORIES: ENA

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Next-generation sequencing facilitates quantitative analysis of rat BMSCs in fast, medium or slow stress relaxation SA hydrogel

Project description:To identify genes and the molecular pathways involved in the regulation of mesenchymal stem cells (MSCs) exposure to various matrix viscoelasticity, we performed RNA-sequence of MSCs in fast stress relaxation (FAST), medium stress relaxation (MEDIUM) and slow stress relaxation (SLOW) matrix.

2021-11-01 | GSE186726 | GEO

Next-generation sequencing facilitates quantitative analysis of rat BMSCs in soft or stiff SA hydrogel

Project description:Next-generation sequencing facilitates quantitative analysis of rat BMSCs in soft or stiff SA hydrogel

| PRJNA752332 | ENA

Next-generation sequencing facilitates quantitative analysis of rat BMSCs in soft or stiff SA hydrogel

Project description:Purpose: To identify genes and the molecular pathways involved in the MSCs response to extracellular matrix stiffness, we performed RNA-sequencing of MSCs which cultured in soft (2 kPa) and stiff (18 kPa) SA hydrogels. Methods: mRNA profiles of MSCs cultured in soft (2 kPa) and stiff (18 kPa) SA hydrogel for 48 h were generated by deep sequencing, in quadruplicate, using Illumina HiSeq 2000. Results: Using an optimized data analysis workflow, we identified 33950 transcripts in MSCs with BWA workflow. Conclusions: Our results present the detailed analysis of MSCs transcriptomes cultured in soft (2 kPa) and stiff (18 kPa) matrix, and found that matrix stiffness dominated multiple mRNA pathways in MSCs.

2021-08-06 | GSE181512 | GEO

Injectable natural polymer hybrid hydrogel targeting aseptic loosening due to wear particle induced osteolysis

Project description:Aseptic loosening represents a significant factor contributing to joint replacement failure, primarily associated with diminished bone formation and heightened osteoclast-induced osteolysis. Here, a natural polymer-based injectable hydrogel that encapsulates irisin protein (referred to as I-OG hydrogel) is reported. The hierarchical cross-linked structure of the I-OG hydrogel confers favorable mechanical properties, desirable self-healing ability, and acceptable injectability and, more importantly, sustains continuous release of the protein at the interface between the bone and implant prosthesis. The I-OG hydrogel effectively fills the gap between the titanium pin and bone tissue, successfully inhibiting aseptic loosening induced by titanium particles, which outcome confirms the occurrence of irisin protein's slow-release process and its inhibitory effect on osteolysis. Mechanistically, our in vitro experiments demonstrated that irisin released from the I-OG hydrogel upregulates the Wnt/β-catenin signaling pathway in bone marrow stromal cells (BMSCs) through integrin αV, while concurrently downregulating the NF-κB (P65) signaling pathway in osteoblasts. These molecular events ultimately promote osteogenic differentiation and inhibit osteoclast activation. Collectively, our findings establish that the I-OG hydrogel effectively counteracts aseptic loosening by resisting osteolysis caused by titanium particles and enhancing periprosthetic bone formation, and offers promising prospects for the treatment of aseptic loosening in prosthetic implants.

2023-06-17 | GSE234863 | GEO

Differences in gene expression between fast and slow muscle or between normal and denervated slow muscles

2014-10-04 | GSE62028 | GEO

Differences in gene expression between fast and slow muscle or between normal and denervated slow muscles

Project description:Fast and slow skeletal muscles show different characteristics and phenotypes. This data obtained from microarray includes the comparison of normal fast plantaris and slow soleus muscles of adult rats. Characters of slow muscle are strongly dependent on the level of muscular activity. Denervation silences the muscular activity. Therefore, we determined the effects of denervation on gene expression in slow soleus muscle of adult rats. Denervation was performed by transection (~5 mm) of left sciatic nerve at the gluteal level. No treatments were made in the normal control rats. Sampling of soleus and/or plantaris was performed in both normal and experimental groups 28 days after the surgery.

2014-10-04 | E-GEOD-62028 | biostudies-arrayexpress

slow/fast muscle with or without amplification

Project description:Comparing the gene expression profiles of slow and fast skeletal muscle (soleus VS FDB) with either amplified RNA (cRNA probes) or original mRNA (cDNA probes). The fidelity of mRNA amplification method in identifying the gene expression profiles of our samples were validated. Keywords: cell type comparison

2007-01-18 | GSE6654 | GEO

Fast and slow muscle proteins in scallops

Project description:We performed the first quantitative proteomics analysis of differences between striated (fast) and catch (slow) adductor muscle in Yesso scallop (Patinopecten yessoensis), with the goal to uncover muscle specific genes and proteins, as well as enzymes of metabolic pathways in fast and slow adductor muscle of scallops. The present findings highlight the functional roles of muscle contractile proteins, calcium signaling pathways, membrane and extracellular matrix proteins, and glycogen metabolism involved in the different contractile and metabolic properties between fast and slow muscles. The present findings will help better understand the molecular basis underlying muscle contraction and its physiological regulation in invertebrates.

2018-10-26 | PXD005166 | Pride

Difference in mRNA between fast and slow type muscle fibers

Project description:In order to invetigate the impact of CpG methylation on gene expression, transcriptomic profiling using microarray were conducted on the fast and slow type myofibers.

2021-07-20 | GSE154721 | GEO

Expression data from young and aged BMSCs

Project description:Bone marrow mesenchymal stem cells (BMSCs) differentiate into various mature cell types, including adipocytes and osteoblasts, which is determined by genetic, molecular mediators and local microenvironment. With age, BMSCs become inclined to undergo differentiation into adipocytes rather than osteoblasts, resulting in an increased number of adipocytes and a decreased number of osteoblasts, causing osteoporosis. The dysregulated the gene expression in BMSCs during aging were analyzed. We used microarrays to detail the global programme of gene expression duing aing in BMSCs.

2018-08-15 | GSE116925 | GEO

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