Project description:Transcriptional profiling of resurrection plant Boea hygrometrica comparing control untreated plants with plants treated with dehydration. Goal was to determine the different effects of desiccation with different rates, and drought acclimation on global gene expression in Boea hygrometrica .
Project description:Gene transcript abundances were analyzed with samples taken from hydrated, moderate dehydration (70% RWC) and desiccated (10% RWC) leaf tissues of resurrection plant species B. hygrometrica by using RNA-Seq. Totally, 9888 genes were identified as differentially expressed genes.The results provided insight for exploring the mechanisms of desiccation tolerance.
Project description:Plants are competent to regenerate new individuals from differentiated tissues under appropriate culture conditions. Although the molecular basis of shoot regeneration has steadily been unraveled, role of DNA methylation in the regulation of plant regeneration capacity remains practically unknown. Here, we established an effective auxin/cytokinin-induced regeneration system of the Chinese resurrection plant Boea hygrometrica through direct organogenesis, and observed that the potential regeneration capacity of leaf explants was gradually decreased with increased age of donor plants. Global transcriptome analysis revealed significant up-regulation of genes required for phenylpropanoid biosynthesis and phytohormone signaling while inhibition of photosynthetic activity in leaf explants during regeneration. Transcriptional changes of positive regulators HY5, STM, EMK and FLA, and negative regulators TSD and CDK involved in plant regeneration, were positively correlated with the regeneration process of B. hygrometrica, implicating their conserved functions across plant species. Comparison of global DNA methylation profiles between expanding young leaves and fully expanded mature leaves with different regeneration capability using whole-genome bisulfite sequencing revealed that increased asymmetrical methylation in mature leaves were predominant distributed in promoter regions, demonstrating their putative inhibitory function for downstream gene expression in B. hygrometrica leaves during maturation. Moreover, the predicted possible DNA methylation control for genes encoding GRCWP1 and BGS40L essential for cell wall architecture, CENL1 controlling extend shoot meristem phases, ANL2 and WRKY75 mediating root hair development, as well as HY5 and two members of ABA signaling components ABF and PP2C also provide new insights into the association of DNA methylation dynamics with regeneration capacity.
Project description:Gene transcript abundances were analyzed with samples taken from desiccated (10% RWC), rehydrated to 50% RWC (RE50% RWC) and rehydrated to 100% RWC (RE100% RWC) leaf tissues of resurrection plant species B. hygrometrica by using RNA-Seq. Totally, 10207 genes were identified as differentially expressed genes.The results provided insight for exploring the mechanisms of desiccation tolerance.
Project description:Gene transcript abundances were analyzed with samples taken from hydrated, moderate dehydration (70% RWC) and desiccated (10% RWC) leaf tissues of resurrection plant species B. hygrometrica by using RNA-Seq. Totally, 9888 genes were identified as differentially expressed genes.The results provided insight for exploring the mechanisms of desiccation tolerance. Examination of mRNA transcript abundances in hydrated, dehydrating to 70% RWC and dehydrating to 10% RWC leaf tissues, for each treatment, three biological replicates were included.
Project description:Transcriptional profiling of resurrection plant Boea hygrometrica comparing control untreated plants with plants treated with dehydration. Goal was to determine the different effects of desiccation with different rates, and drought acclimation on global gene expression in Boea hygrometrica . 8 conditions comparison: untreated plants vs. plants treated with rapid dehydration for 2h (RWC~80%) and 48h (RWC<10%), untreated plants vs.plants treated with slow dehydration for 5d (RWC~80%) and 14d (RWC<10%), untreated plants vs. drought acclimated plants treated with rapid dehydration for 2h (RWC~80%) and 48h (RWC<10%). Biological replicates: 3 control replicates, 3 treated replicates.
