Project description:To reveal the changes of miRNAs levels in HCT116 cells with METTL14 overexpression, we employed miRNA microarray expression profiling in HCT116 human colon cancer cells stably expressing control vector or METTL14.
Project description:In order to define the cohort of genes whose expression is regulated by METTL14 in p53 wild-type colorectal cancer, we constructed HCT116 cells stably infected with lentivirus carrying control shRNA (sh_NC) or METTL14 shRNA (sh_MET). Subsequently, total RNA was isolated and then performed transcriptome RNA sequence.