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Preliminary investigation of the effect of transfected HBx on the expression pattern of circRNAs in hepatocellular carcinoma

ABSTRACT: Preliminary investigation of the effect of transfected HBx on the expression pattern of circRNAs in hepatocellular carcinoma

PROVIDER: PRJNA776640 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16646685_1.fastq.gz	Fastqsanger.gz
	SRR16646685_2.fastq.gz	Fastqsanger.gz
	SRR16646686_1.fastq.gz	Fastqsanger.gz
	SRR16646686_2.fastq.gz	Fastqsanger.gz

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Preliminary investigation of the effect of transfected HBx on the expression pattern of circRNAs in hepatocellular carcinoma

Project description:Preliminary investigation of the effect of transfected HBx on the expression pattern of circRNAs in hepatocellular carcinoma

2022-10-31 | GSE186884 | GEO

Wild-type versus HBx-transfected mive

Project description:Hepatitis B virus (HBV) has been clearly recognized as an etiological factor for hepatocellular carcinoma (HCC). HBV encodes the potentially oncogenic HBx protein. We aimed to elucidate the molecular mechanism of HCC caused by HBx and to discover the biomarker related to HCC by HBx. Three experimental groups, 3, 9 and 13 month aged HBx Tg mice and age matched normal wild type B6 mouse which have same background of HBx Tg mice were used to find differentially expressed genes during HCC. Keywords: Genetic modification 3-month-old, 9-month-old, 13-month-old wild type B6 mice vs 3-month-old, 9-month-old, 13-month-old HBx transfected mice; Biological replicates at each timepoint; 9 controls vs 9 HBx-mice

2010-06-19 | E-GEOD-12723 | biostudies-arrayexpress

Wild-type versus HBx-transfected mive

2008-09-11 | GSE12723 | GEO

Differential gene expression profiling of MIHA cells transfected with C-terminal truncated HBx mutants

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Differential gene expression profiling of MIHA cells transfected with C-terminal truncated HBx mutants

Project description:To compare the differentially expressed transcriptomes between MIHA cells transfected with empty vector control or different C-terminal truncated HBx mutants (14 or 35 amino acid carboxyl-terminal truncation - i.e. d14 and d35) mRNA profiles of MIHA cells stably overexpressing empty vector control or different C-terminal truncated HBx mutants (delta 14 and delta 35) were generated by PolyA mRNA sequencing using Illumina HiSeq 1500 platform

2016-08-02 | E-GEOD-71993 | biostudies-arrayexpress

Effect of overexpressing of hepatitis B protein x (HBx) on the expression of circular RNAs (circRNAs) in HepG2 cells

Project description:CircRNA-sequencing in HBx-overexpressing (HBx-oe) and NC (negative control) HepG2 cells

2022-10-18 | GSE215232 | GEO

Expression data from Hela cells stably expressing the wild-type HBx and the four mutant HBx

Project description:In large cohort studies, several HBx mutants were identified as independent risk factors for HCC risk.In order to study the carcinogenic mechanism between different mutants, HeLa cell lines expressing wild type and different mutated HBx proteins were constructed. We used microarrays to describe gene expression in HeLa cells stably expressing the wild-type HBx and the four mutant HBx, and to identify distinct classes of up-regulated genes.

2022-03-02 | GSE179126 | GEO

Identification of substrates of the CRL4-HBx E3 ubiquitin ligase

Project description:Hepatitis B Virus (HBV) remains a major public health problem, and is a major cause liver cancer worldwide. HBV infection in vivo requires the function of the HBx protein, which facilitates expression of viral genes from the episomal genome by an unknown mechanism. Evidence suggests that HBx functions as a targeting subunit for the CRL4 E3 ubiquitin ligase, redirecting the complex to target and degrade an unknown host restriction factor. We used substrate trapping proteomics to search for ubiquitylation substrates of HBx. We used MLN4924 to block CRL activity and stabilize interactions between HBx and its substrates. We then performed APMS to identify bound proteins and potential substrates.

2016-09-29 | PXD004762 | Pride

HBX-HepG2 versus Control-HepG2

Project description:Mature microRNA expression profiling of HBx-expressing HepG2 cells versus Control cells under UV stress The mature microRNA expression profile of HBx-expressing HepG2 cells versus control cells in three independent biological repeated experiments. This study focuses only on human sequences. The complete data matrix is linked below as a supplementary file.

2010-05-16 | E-GEOD-15554 | biostudies-arrayexpress

HBx increases chromatin accessibility and ETV4 expression to regulate Dishevelled-2 and promote HCC progression

Project description:Purpose: The goals of this study are to invsetigate the primary biological process of HBx. Methods: Briefly, samples (L02 cells transfected with pcDNA4.0-vector or pcDNA4.0-HBx) were used to extract total RNAs for quality control and RNA-seq loading samples. RNA-seq was performed as DGE on an Illumina platform and 50 bp paired-end reads were generated (Guangzhou Epibiotek Co.,Ltd.). Results: Negative regulation of transferase activity was identified as the primary biological process according to the gene ontology (GO) analysis. TNF signaling pathway was identified as the primary biological processes according to the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis . DNA binding transcription factor activity was identified as the primary biological processes according to the gene set enrichment analysis (GSEA). Conclusions: RNA-seq in L02 cell lines revealed that HBx mainly regulated the DNA binding transcription factor activity.

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