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Identification of genes regulated by acute overexpression of Yox1 and Yhp1 in S. cerevisiae

ABSTRACT: Identification of genes regulated by acute overexpression of Yox1 and Yhp1 in S. cerevisiae

PROVIDER: PRJNA777887 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16771321.fastq.gz	Fastqsanger.gz
	SRR16771322.fastq.gz	Fastqsanger.gz
	SRR16771323.fastq.gz	Fastqsanger.gz
	SRR16771324.fastq.gz	Fastqsanger.gz
	SRR16771325.fastq.gz	Fastqsanger.gz

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Identification of genes regulated by acute overexpression of Yox1 and Yhp1 in S. cerevisiae

Project description:We report gene expression data for strains that overexpress the transcriptional repressors Yox1 or Yhp1 from a synthetic, estradiol-inducible promoter. Gene expression was also measured in strains that overexpress unphosphorylatable alleles of either Yox1 or Yhp1, to determine whether blocking phosphorylation of these proteins by Cdk1 changes the spectrum of regulated genes. Finally, gene expression was measured in corresponding control strains in which Yox1/Yhp1 proteins were expressed from their endogenous promoters. These data demontsrate that Yox1 is a stronger repressor than Yhp1, although they regulate the same genes. In addition, phosphodeficient alleles of each TF regulate the same genes as the wild type proteins.

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