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Prospermatogonia transcriptome changes in Taf4b-deficient male mice

ABSTRACT: Prospermatogonia transcriptome changes in Taf4b-deficient male mice

PROVIDER: PRJNA778416 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16832600_1.fastq.gz	Fastqsanger.gz
	SRR16832600_2.fastq.gz	Fastqsanger.gz
	SRR16832601_1.fastq.gz	Fastqsanger.gz
	SRR16832601_2.fastq.gz	Fastqsanger.gz
	SRR16832602_1.fastq.gz	Fastqsanger.gz

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Prospermatogonia transcriptome changes in Taf4b-deficient male mice

Project description:Taf4b-deficient male mice are initially sub-fertile and become infertile due to a depletion of the spermatogonial stem cell (SSC) reserve. During embryonic time points, significantly reduced numbers of germ cells have been observed in the Taf4b-deficient male gonad and previous research has shown that Taf4b mRNA expression peaks at E15.5. Therefore, we hypothesized that TAF4b, as part of TFIID, regulates cell cycle and SSC gene programs. We performed RNA-seq to examine gene expression changes in E14.5 and E16.5 male Taf4b-wildtype, Taf4b-heterozygous, and Taf4b-deficient germ cells

2023-10-04 | GSE188351 | GEO

CUT&RUN of TAF4b and H3K4me3 in E16.5 prospermatogonia

Project description:Taf4b-deficient male mice are initially sub-fertile and become infertile due to a depletion of the spermatogonial stem cell (SSC) reserve. During embryonic time points, significantly reduced numbers of germ cells have been observed in the Taf4b-deficient male gonad and previous research has shown that Taf4b mRNA expression peaks at E15.5. Therefore, we hypothesized that TAF4b, as part of TFIID, regulates cell cycle and SSC gene programs. However, the direct targets of TAF4b have not been thoroughly explored. Therefore, we performed Cleavage Under Targets and Release Using Nuclease (CUT&RUN) in E16.5 mouse prospermatogonia.

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Oocyte transcriptome changes in Taf4b-deficient female mice [E14.5]

Project description:Research has shown that Taf4b-deficient female mice display excessive perinatal germ cell death, delayed germ cell cyst breakdown, and increased chromosome asynapsis. Therefore, we hypothesized that TAF4b, as part of TFIID, regulates oogenesis and meiotic gene programs. However, the transcriptomic effects of Taf4b-deficiency and how this may lead to the infertility we observe in mice has not yet been studied. Therefore, we performed RNA-seq to examine gene expression changes in E14.5 female Taf4b-wildtype, Taf4b-heterozygous, and Taf4b-deficient germ cells

2022-01-26 | GSE174364 | GEO

Oocyte transcriptome changes in Taf4b-deficient female mice [E16.5]

Project description:Research has shown that Taf4b-deficient female mice display excessive perinatal germ cell death, delayed germ cell cyst breakdown, and increased chromosome asynapsis. Therefore, we hypothesized that TAF4b, as part of TFIID, regulates oogenesis and meiotic gene programs. However, the transcriptomic effects of Taf4b-deficiency and how this may lead to the infertility we observe in mice has not yet been studied. Therefore, we performed RNA-seq to examine gene expression changes in E16.5 female Taf4b-heterozygous and Taf4b-deficient germ cells

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Oocyte transcriptome changes in Taf4b-deficient female mice

Project description:Oocyte transcriptome changes in Taf4b-deficient female mice

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CUT&RUN of TAF4b and H3K4me3 in E16.5 prospermatogonia

Project description:CUT&RUN of TAF4b and H3K4me3 in E16.5 prospermatogonia

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Oocyte transcriptome changes in Taf4b-deficient female mice [E16.5]

Project description:Oocyte transcriptome changes in Taf4b-deficient female mice [E16.5]

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Oocyte transcriptome changes in Taf4b-deficient female mice [E14.5]

Project description:Oocyte transcriptome changes in Taf4b-deficient female mice [E14.5]

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CUT&RUN of TAF4b and H3K4me3 in E16.5 oocytes

Project description:Research has shown that Taf4b-deficient female mice display excessive perinatal germ cell death, delayed germ cell cyst breakdown, and increased chromosome asynapsis. Therefore, we hypothesized that TAF4b, as part of TFIID, regulates oogenesis and meiotic gene programs. However, the direct targets of TAF4b have not been thoroughly explored. Therefore, we performed Cleavage Under Targets and Release Using Nuclease (CUT&RUN) in E16.5 mouse oocytes.

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Single-Cell Transcriptome Analysis of Rb1 Conditional Knockout Prospermatogonia

Project description:Prospermatogonia, precursors to the mature male germline, undergo a period of sustained quiescence during late fetal and early postnatal development. The purpose of this mitotic arrest is not fully understood. Therefore, utilizing conditional ablation of retinoblastoma protein 1 (Rb1) in mice which blocks entry into quiescence, we analyzed prospermatogonia at the cusp of mitotic arrest at embryonic day 14.5. Prospermatogonia from heterozygous controls or conditional knockout males were isolated via fluorescence-activated cell sorting and profiled via single-cell RNA-sequencing (scRNA-seq). Outcomes of scRNA-seq analysis revealed that a metabolic shift to oxidative phosphorylation and meiotic inhibition are disrupted in prospermatogonia that fail to initiate quiescence. Collectively, these findings and others suggest that biosensing mechanisms eliminate prospermatogonia that fail to mitotically arrest and that key layers of programming are applied during quiescence to ensure proper fate specification.

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