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Transcriptome profiling of hESC-derived endoderm progenitors by single-cell RNA-seq

ABSTRACT: Transcriptome profiling of hESC-derived endoderm progenitors by single-cell RNA-seq

PROVIDER: PRJNA778540 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16848290.fastq.gz	Fastqsanger.gz
	SRR16848291.fastq.gz	Fastqsanger.gz
	SRR16848292.fastq.gz	Fastqsanger.gz
	SRR16848293.fastq.gz	Fastqsanger.gz
	SRR16848294.fastq.gz	Fastqsanger.gz

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Transcriptome profiling of hESC-derived endoderm progenitors by RNA-seq

Project description:Transcriptome profiling of hESC-derived endoderm progenitors by RNA-seq. RNA-seq analysis during the stepwise progression of hESC toward the pancreatic progenitors, at five defined stages of differentiation and expansion: hESC, ADE, VFG.p3, VFG.p6. PE.

2022-11-15 | GSE185670 | GEO

Transcriptome profiling of hESC-derived endoderm progenitors by RNA-seq

Project description:Transcriptome profiling of hESC-derived endoderm progenitors by RNA-seq

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Transcriptome profiling of hESC-derived endoderm progenitors by single-cell RNA-seq

Project description:In this study, we characterised transcriptomes of hESC-derived transient anterior definitive endoderm (ADE) and expanding ventral foregut (VFG) culture by single-cell sequencing. We also examined the effect of BMP4 withdrawal and FGF2 stimulation in the VFG culture.

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Single cell transcriptome profiling of mouse and hESC-derived pancreatic progenitors

Project description:Human embryonic stem cells (hESCs) are a potential unlimited source of insulin-producing β-cells for diabetes treatment. A greater understanding of how β-cells form during embryonic development will improve current hESC differentiation protocols. As β-cells are formed from NEUROG3-expressing endocrine progenitors, this study focused on characterizing the single-cell transcriptomes of mouse and hESC-derived endocrine progenitors. To do this, 7,223 E15.5 and 6,852 E18.5 single cells were isolated from Neurog3-Cre; Rosa26mT/mG embryos, allowing for enrichment of endocrine progenitors (yellow; tdTomato + EGFP) and endocrine cells (green; EGFP). From a NEUROG3-2A-eGFP CyT49 hESC reporter line (N5-5), 4,497 hESC-derived endocrine progenitor cells were sequenced. Differential expression analysis reveals enrichment of markers that are consistent with progenitor, endocrine, or novel cell-state populations. This study characterizes the single-cell transcriptomes of mouse and hESC-derived endocrine progenitors and serves as a resource (https://lynnlab.shinyapps.io/embryonic_pancreas/) for improving the formation of functional β-like cells from hESCs.

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Project description:Single cell transcriptome profiling of mouse and hESC-derived pancreatic progenitors

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3' RNA-Seq (3'SEQ) analysis gene expression profiling at G1-S transition in human embryonic stem cells (hESCs) and hESC-derived endoderm cells

Project description:In this report, we examine transcripts on a genome-wide level between the synchronized cell cycles of hESCs and hESC-derived endoderm cells. We found 10347, 10299 and 10362 genes expressed in the G1, G1/S, and S phase of hESCs, respectively; 10333, 10227 and 10215 genes expressed at the G1, G1/S and S phase of derived endoderm. We compared the transcriptome between these data sets and identified genes with differentiated phase expression between hESCs and hESC-derived endoderm cells. Examination of the transcriptome at the G1-S transition in hESCs compared to hESC-derived endoderm cells.

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Project description:Human embryonic stem cells (hESCs) are a potential unlimited source of insulin-producing ? cells for diabetes treatment. A greater understanding of how ? cells form during embryonic development will improve current hESC differentiation protocols. All pancreatic endocrine cells, including ? cells, are derived from Neurog3-expressing endocrine progenitors. This study characterizes the single-cell transcriptomes of 6,905 mouse embryonic day (E) 15.5 and 6,626 E18.5 pancreatic cells isolated from Neurog3-Cre; Rosa26mT/mG embryos, allowing for enrichment of endocrine progenitors (yellow; tdTomato + EGFP) and endocrine cells (green; EGFP). Using a NEUROG3-2A-eGFP CyT49 hESC reporter line (N5-5), 4,462 hESC-derived GFP+ cells were sequenced. Differential expression analysis revealed enrichment of markers that are consistent with progenitor, endocrine, or previously undescribed cell-state populations. This study characterizes the single-cell transcriptomes of mouse and hESC-derived endocrine progenitors and serves as a resource (https://lynnlab.shinyapps.io/embryonic_pancreas) for improving the formation of functional ?-like cells from hESCs.

| S-EPMC6294286 | biostudies-literature

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