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Two zinc finger proteins with functions in m6A writing interact with HAKAI

ABSTRACT: Two zinc finger proteins with functions in m6A writing interact with HAKAI

PROVIDER: PRJNA779683 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14570256_1.fastq.gz	Fastqsanger.gz
	SRR14570256_2.fastq.gz	Fastqsanger.gz
	SRR14570257_1.fastq.gz	Fastqsanger.gz
	SRR14570257_2.fastq.gz	Fastqsanger.gz
	SRR14570258_1.fastq.gz	Fastqsanger.gz

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Two zinc finger proteins with functions in m6A writing interact with HAKAI

Project description:The methyltransferase complex (m6A writer), which catalyzes the deposition of N6-methyladenosine (m6A) in mRNAs, is highly conserved across most eukaryotic organisms, but its components and interactions between them are still far from fully understood. Using in vivo interaction proteomics, two HAKAI-interacting zinc finger proteins, HIZ1 and HIZ2, were discovered as novel components of the Arabidopsis m6A writer complex. HAKAI is required for the interaction between HIZ1 and MTA. Whilst HIZ1 knockout plants have normal levels of m6A, plants in which it is overexpressed show reduced methylation. In addition, HIZ1 was found to be involved in root hair development upon auxin transport inhibition in a HAKAI-dependent manner. Mutant plants lacking HIZ2 are viable but have an 85% reduction in m6A abundance and show severe developmental defects. Our findings suggest that HIZ1 appears to be a HAKAI-dependent negative regulator of m6A deposition and HIZ2 is a novel and essential member of the Arabidopsis m6A writer complex.

2022-02-09 | PXD026287 | Pride

Two novel proteins with opposing m6A writing functions interact with HAKAI [meRIP-seq]

Project description:Using in vivo interaction proteomics, two HAKAI-interacting zinc finger proteins, HIZ1 and HIZ2, were discovered as novel components of the Arabidopsis m6A writer complex. HAKAI is required for the interaction between HIZ1 and MTA. Whilst HIZ1 knockout plants have normal levels of m6A, plants in which it is overexpressed show reduced methylation. In addition, HIZ1 was found to be involved in root hair development upon auxin transport inhibition in a HAKAI-dependent manner. Mutant plants lacking HIZ2 are viable but have an 85% reduction in m6A abundance and show severe developmental defects. Our findings suggest that HIZ1 appears to be a HAKAI-dependent negative regulator of m6A deposition and HIZ2 is a novel and essential member of the Arabidopsis m6A writer complex.

2022-01-20 | GSE174573 | GEO

Two novel proteins with opposing m6A writing functions interact with HAKAI [RNA-seq]

2022-01-21 | GSE188610 | GEO

Two novel proteins with opposing m6A writing functions interact with HAKAI [RNA-seq]

Project description:Two novel proteins with opposing m6A writing functions interact with HAKAI [RNA-seq]

| PRJNA779686 | ENA

Two novel proteins with opposing m6A writing functions interact with HAKAI [meRIP-seq]

Project description:Two novel proteins with opposing m6A writing functions interact with HAKAI [meRIP-seq]

| PRJNA730562 | ENA

Two zinc finger proteins with functions in m<sup>6</sup>A writing interact with HAKAI.

Project description:The methyltransferase complex (m6A writer), which catalyzes the deposition of N6-methyladenosine (m6A) in mRNAs, is highly conserved across most eukaryotic organisms, but its components and interactions between them are still far from fully understood. Here, using in vivo interaction proteomics, two HAKAI-interacting zinc finger proteins, HIZ1 and HIZ2, are discovered as components of the Arabidopsis m6A writer complex. HAKAI is required for the interaction between HIZ1 and MTA (mRNA adenosine methylase A). Whilst HIZ1 knockout plants have normal levels of m6A, plants in which it is overexpressed show reduced methylation and decreased lateral root formation. Mutant plants lacking HIZ2 are viable but have an 85% reduction in m6A abundance and show severe developmental defects. Our findings suggest that HIZ2 is likely the plant equivalent of ZC3H13 (Flacc) of the metazoan m6A-METTL Associated Complex.

| S-EPMC8891334 | biostudies-literature

Two zinc finger proteins with functions in m6A writing interact with HAKAI

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-01-21 | GSE188611 | GEO

Hakai

Project description:Drosophila melanogaster Hakai, Hakai, is differentially expressed in 7 experiment(s);

| FBgn0032812 | ExpressionAtlas

Hakai

Project description:Drosophila melanogaster Hakai, Hakai, is expressed in 2 baseline experiment(s);

| FBgn0032812 | ExpressionAtlas

Multiparameter functional diversity of human C2H2 zinc finger proteins [RNA-Seq]

Project description:C2H2 zinc finger proteins represent the largest and most enigmatic class of human transcription factors. Their C2H2 arrays are highly variable, indicating that most will have unique DNA binding motifs. However, most of the binding motifs have not been directly determined. We have determined the binding sites and motifs of 119 C2H2 zinc finger proteins and the expression pattern of 80 cell lines overexpressing C2H2 zinc finger proteins in order to study the role of C2H2 zinc finger proteins in gene regulation. We expressed GFP-tagged C2H2-ZF proteins in stable transgenic HEK293 cells. Total RNA was isolated using Trizol and sequencing libraries were constructed using TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold or TruSeq RNA Library Preparation Kit v2.

2016-10-25 | E-GEOD-76495 | biostudies-arrayexpress

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