Project description:To identify differentially expressed genes in tumor tissues, several human cancer tissues (hypopharyngeal squamous cell carcinoma, maxillary sinus squamous cell carcinoma, and renal cell carcinoma) were subjected to Agilent whole genome microarrays. A total of nine pairs of primary hypopharyngeal squamous cell carcinoma samples and adjacent normal mucosa were obtained from patients who underwent tumor resection at Chiba University Hospital (Chiba, Japan). A total of seven pairs of primary maxillary sinus squamous cell carcinoma samples and adjacent normal mucosa were obtained from patients who underwent tumor resection at Chiba University Hospital (Chiba, Japan). A total of five pairs of renal cell carcinoma samples and adjacent normal tissues were obtained from patients who underwent tumor resection at Kagoshima University Hospital (Kagoshima, Japan). The Ethics Committee of Chiba University and the Bioethics Committee of Kagoshima University approved our study, and informed consent was obtained from all patients for use of their tissue samples and clinical data. The tissue samples were immediately frozen in liquid nitrogen and stored at -80°C until use.

2012-11-12 | E-GEOD-36951 | biostudies-arrayexpress

Isolation, identification and osteogenic capability analysis of mesenchymal stem cells derived from different layers of human maxillary sinus membrane

Project description:Mesenchymal stem cells (MSCs) from different tissue sources have different biological processes and differentiation abilities. Although previous studies confirm that MSCs derived from human maxillary sinus membrane (hMSM) can be cultured in vitro, it cannot confirm whether they belong to the same type and possess the same differentiation ability. In this study, lamina propria layer derived MSCs (hLMSCs) and periosteum layer derived MSCs (hPMSCs) from hMSM are isolated and expanded using explant cell culture method and the biological characteristics of hLMSCs and hPMSCs are compared using RNA sequencing. According to RNA sequencing and subsequent functional verification, hLMSCs and hPMSCs are identified as CD171+/CD90+ and CD171-/CD90+ phenotypes separately. This study suggests that two different types of MSCs can be identified and isolated from hMSMs. Both cells serve as good MSCs potential candidates for bone regeneration. It also indicates that hLMSCs and hPMSCs are valuable in vitro tools for preclinical studies and future exploration of the origin osteogenesis machenism in maxillary sinus region.

2024-06-30 | GSE240641 | GEO

Identification of a Facial Enhancer

Project description:We generated a genome-wide map of candidate enhancers from the maxillary arch (primordium for the upper jaw) of mouse embryos Examination of histone modification H3K27ac (distinguishes active enhancers from inactive enhancer elements) in the maxillary arch tissue

2014-09-02 | E-GEOD-55106 | biostudies-arrayexpress

Single-cell transcriptome profiling reveals developmental trajectory and regulatory networks of mouse maxillary prominence [ATAC-seq]

Project description:The formation of maxillary prominence in vertebrates involves precisely coordinated migration and differentiation of neural crest cells. However, the regulatory factors and networks underpinning such an intricate process has not been fully elucidated. Here we combined bulk and single-cell RNA-Seq to comprehensively characterize the transcriptome dynamics during mouse maxillary development from embryonic day (E) 10.5 to 12.5, a critical period when a multitude of facial tissues start to emerge. We identified multiple cell populations that represent different developmental transition states and inferred the developmental trajectory of maxillary mesenchyme, revealing that a developmental bifurcation toward either an osteochondrogenic fate or a non-osteochondrogenic fate occurs at E11.5. We further deduced the core transcriptional regulators and gene regulatory networks associated with the maxillary cell fate transitions. Moreover, we revealed transcriptional regulators that are linked to dynamic changes in chromatin accessibility during maxillary development. Collectively, our study for the first time characterized the maxillary developmental process at single cell resolution, providing rich resources and important insights for achieving a systems level understanding of craniofacial morphogenesis and abnormality.

2023-11-30 | GSE161130 | GEO

Identification of a Facial Enhancer

Project description:We generated a genome-wide map of candidate enhancers from the maxillary arch (primordium for the upper jaw) of mouse embryos

2014-09-02 | GSE55106 | GEO

Identification of an LHX target cis-regulatory element through LHX6 ChIP-seq.

Project description:We performed chromatin immunoprecipitation (ChIP) followed by high-throughput sequencing (seq) from mouse E11.5 maxillary arches using anti-LHX6 antibody to identify LHX target cis-regulatory elements.

2017-07-28 | GSE71497 | GEO

Single-cell transcriptome profiling reveals developmental trajectory and regulatory networks of mouse maxillary prominence [RNA-seq]

2023-11-30 | GSE161126 | GEO

Comparison of DNA methyltransferase-1 (Dnmt1) deleted urogenital sinus epithelium compared to wildtype urogenital sinus epithelium

Project description:Dnmt1 is an important regulator of tissue development and differentiation. To assess the effects of epithelium Dnmt1 deletion in the developing urogenital sinus (precursor of the urethra and prostate in males), we isolated urogenital sinus epithelial tissue from Dnmt1 deleted mouse embryos and wildtype mouse embryos. The transcriptomes were analyzed by RNA-seq

2018-12-31 | GSE121086 | GEO

Project description:Single cell RNA sequencing of mouse maxillary process

| PRJNA891206 | ENA