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Generation of human iPS cell-derived mesenchymal cells capable of forming alveolar organoids [bulkRNA-Seq]

ABSTRACT: Generation of human iPS cell-derived mesenchymal cells capable of forming alveolar organoids [bulkRNA-Seq]

PROVIDER: PRJNA780481 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16947426_1.fastq.gz	Fastqsanger.gz
	SRR16947426_2.fastq.gz	Fastqsanger.gz
	SRR16947427_1.fastq.gz	Fastqsanger.gz
	SRR16947427_2.fastq.gz	Fastqsanger.gz
	SRR16947428_1.fastq.gz	Fastqsanger.gz

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Generation of human iPS cell-derived mesenchymal cells capable of forming alveolar organoids

Project description:Generation of human iPS cell-derived mesenchymal cells capable of forming alveolar organoids

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Generation of human iPS cell-derived mesenchymal cells capable of forming alveolar organoids [scRNA-Seq]

Project description:Generation of human iPS cell-derived mesenchymal cells capable of forming alveolar organoids [scRNA-Seq]

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Generation of human iPS cell-derived mesenchymal cells capable of forming alveolar organoids [bulkRNA-Seq]

Project description:We developed a method of generating human induced pluripotent stem cell (iPSC)-derived mesenchymal cells (iMES) that were able to induce AT1 and AT2 epithelial lineage cells in organoids. Transcriptomic analysis of human fetal lung fibroblasts (HFLF), human dermal fibroblasts (HDF), and their corresponding iMES revealed that iMES harbor characteristics of lung distal tip mesenchyme.

2022-09-16 | GSE188822 | GEO

Generation of human iPS cell-derived mesenchymal cells capable of forming alveolar organoids [scRNA-Seq]

Project description:We have developed a method to generate human induced pluripotent stem cell (iPSC)-derived mesenchymal cells (iMES) that were able to induce AT1 and AT2 epithelial cells within their organoids (iMES-AO). Single-cell transcriptome analysis comparing iMES-AO with our previously reported alveolar organoids using human fetal lung fibroblasts delineated not only differences in composition of epithelial lineages but also distinctive mesenchymal lineages.

2022-09-16 | GSE188823 | GEO

Molecular Insights into Non-teratoma-forming Induced Pluripotent Stem Cells

Project description:Human induced pluripotent stem (iPS) cells are capable of differentiating into derivatives of the three embryonic germ layers both in vitro and in vivo. To date the the molecular differences between teratoma-forming cells and non-teratoma-forming cells has not been analysed. A cell line, B1, bears typical ES cell-like morphology, expression of pluripotency-associated genes, and in vitro pluripotency capacity, but fails to form teratomas after subcutaneously injected into immune-deficient mice based on histological analysis. Besides histological analysis, we characterized the tumors derived from line B1, and teratomas derived from bona fida iPS and ES (line H1) cells respectively, using microarray-based gene expression analysis. The expression levels of pluripotency-associated markers in B1 cells were comparable to that in iPS and ES cells, while the complexity of tissue expression commitment was decreased upon spontaneous differentiation of B1 cells as compared to iPS and ES cells. Total RNA obtained from HFF1 (human foreskin fibroblast) cells, line B1, iPS-A4, iPS-B4 and ES (line H1) cells, and their derived tumors in immune-deficient mice.

2012-04-29 | E-GEOD-31970 | biostudies-arrayexpress

Generation of human iPS cell-derived mesenchymal cells capable of forming alveolar organoids

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-09-16 | GSE188825 | GEO

Molecular Insights into Non-teratoma-forming Induced Pluripotent Stem Cells

2012-04-30 | GSE31970 | GEO

Kidney organoids are capable of forming Wilms-like tumors, but not teratomas

Project description:Kidney organoids are capable of forming Wilms-like tumors, but not teratomas

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ACE2 knockout hinders SARS-CoV-2 propagation in iPS cell-derived airway and alveolar epithelial cells

Project description:Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, continues to spread around the world with serious cases and deaths. It has also been suggested that different genetic variants in the human genome affect both the susceptibility to infection and severity of disease in COVID-19 patients. Angiotensin-converting enzyme 2 (ACE2) has been identified as a cell surface receptor for SARS-CoV and SARS-CoV-2 entry into cells. The construction of an experimental model system using human iPS cells would enable further studies of the association between viral characteristics and genetic variants. Airway and alveolar epithelial cells are cell types of the lung that express high levels of ACE2 and are suitable for in vitro infection experiments. Here, we show that human iPS cell-derived airway and alveolar epithelial cells are highly susceptible to viral infection of SARS-CoV-2. Using gene knockout with CRISPR-Cas9 in human iPS cells we demonstrate that ACE2 plays an essential role in the airway and alveolar epithelial cell entry of SARS-CoV-2 in vitro. Replication of SARS-CoV-2 was strongly suppressed in ACE2 knockout (KO) lung cells. Our model system based on human iPS cell-derived lung cells may be applied to understand the molecular biology regulating viral respiratory infection leading to potential therapeutic developments for COVID-19 and the prevention of future pandemics.

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Single-Cell RNA Sequencing Reveals Human iPS Cell-derived Alveolar Type 1 Cells in Alveolar Organoids

Project description:Single-Cell RNA Sequencing Reveals Human iPS Cell-derived Alveolar Type 1 Cells in Alveolar Organoids

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