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Gene expression data of SPC25 KD in HCCLM3 cell line

ABSTRACT: Gene expression data of SPC25 KD in HCCLM3 cell line

PROVIDER: PRJNA780555 | ENA |

REPOSITORIES: ENA

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Gene expression data of SPC25 KD in HCCLM3 cell line

Project description:Recently, SPC25 was shown to be overexpressed in HCC, but the mechanisms whereby this occurs remain unknown. In this study, we found that SPC25 was significantly overexpressed in HCC tissues, with further analysis revealing that elevated SPC25 expression was associated with HCC prognosis and metastasis. To further explore the mechanism of SPC25 in HCC, we performed a cDNA microarray in SPC25 knockdown HCCLM3 cells.

2022-02-28 | GSE188881 | GEO

Effect of CTHRC1 knockdown on gene expression of HCCLM3 cells

Project description:Collagen triple helix repeat containing 1 (CTHRC1) has been found to be up-regulated in many human solid tumors. In this study, we investigated the changes of gene expression by comparing CTHRC1-siRNA and Scramble-siRNA control in hepatocellular carcinoma cell line HCCLM3, which has high expression levels of CTHRC1. Differential gene expression was assessed by Affymetrix microarray experiments for 2 samples: CTHRC1-siRNA-treated HCCLM3 cells and Scramble-siRNA-treated HCCLM3 cells.

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Gene expression data of PFKFB3 KD in SMMC7721 cell line

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Effect of CTHRC1 knockdown on gene expression of HCCLM3 cells

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Gene expression analysis of TRIM5 KD in the THP1 cell line

Project description:We had evidence that TRIM5 regulates signal transduction, specifically NFkB and MAPK pathways. To test the role of endogenous TRIM5 we used the myelomonocytic leukemia cell line THP1. These cells were transduced with a lentiviral vector that delivers a miRNA engineered to knockdown TRIM5. The vector also encoded a puromycin-resistance cassette and transduced cells were selected in poold with puromycin. As a control, cells were transduced with a vector targeting luciferase instead of TRIM5. After selection in puromycin, the TRIM5 KD and Luciferase KD cells were differentiated into macrophages by treatment with PMA. 3 days later, RNA was harvested for analysis.

2010-11-02 | E-GEOD-25041 | biostudies-arrayexpress

Searching for target genes of miR-429 in HCCLM3 cells

Project description:miRNAs are an class of small noncoding RNAs and about 21-25 nucleotides in length. miRNAs inhibit the translation or induce mRNA degradation by binding to the 3’ UTR of target mRNAs and have been identified as the tumor promoters or suppressors regulating the progression of cancers. miR-429, which is a member of an evolutionarily conserved family of miRNAs that includes miR-200b, miR-200a, miR-200c and miR-141, is expressed in various epithelial tissues. Our goal is to search the possible target genes of miR-429 in human liver cancer cell line HCCLM3. We analyzed possible target genes of miR-429 by identifying the shared genes among the down-regulated genes in epithelial cell adhesion molecule (EpCAM) negative HCCLM3 cells which treated with miR-429 mimics and the up-regulated genes in EpCAM positive HCCLM3 cells which treated with Antagomir-429.

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Neurospora crassa strain:NCU01888-KD

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