Blastocladiella emersonii
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA78059 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| SRR12507012_1.fastq.gz | Fastqsanger.gz | |||
| SRR12507012_2.fastq.gz | Fastqsanger.gz | |||
| SRR12507013.fastq.gz | Fastqsanger.gz | |||
| SRR12507014.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 8 |
Similar Datasets
Project description:Global gene expression analysis during sporulation of the aquatic fungus Blastocladiella emersonii
| PRJNA121519 | ENA
Project description:Blastocladiella emersonii life cycle presents a number of drastic biochemical and morphological changes, mainly during two cell differentiation stages: the germination and the sporulation. To investigate the transcriptional changes taking place during the sporulation phase, which culminates with the production of the zoospores, motile cells responsible for the dispersal of the fungus, microarray experiments were performed.
2009-10-28 | GSE18718 | GEO
Project description:Gene expression during germination in the Chytridiomycete Blastocladiella emersonii
| PRJNA110871 | ENA
Project description:Response of Blastocladiella emersonii to hypoxia, iron deprivation and geldanamycin treatment followed by hypoxia.
| PRJNA118805 | ENA
Project description:Gene expression in response to stresses in the Chytridiomycete Blastocladiella emersonii.
| PRJNA97597 | ENA
Project description:Blastocladiella emersonii EST project
| PRJNA41385 | ENA
Project description:Due to its aquatic saprophytic lifestyle, the fungus Blastocladiella emersonii seems to be adapted to low aerated environments and thus can be an interesting model of study of the hypoxic stress response. Iron deprivation, a hypoxia-mimicking stimulus due to HIF-1 stabilization, and geldanamycin, that causes HIF-1 depletion by HSP90 inhibition, are helpful parameters to find an evidence of an oxygen-dependent regulatory mechanism in B. emersonii similar to the metazoan HIF-1 pathway.
2009-08-07 | GSE17524 | GEO
Project description:BackgroundExposure of cells to environmental stress conditions can lead to the interruption of several intracellular processes, in particular those performed by macromolecular complexes such as the spliceosome.ResultsDuring nucleotide sequencing of cDNA libraries constructed using RNA isolated from B. emersonii cells submitted to heat shock and cadmium stress, a large number of ESTs with retained introns was observed. Among the 6,350 ESTs obtained through sequencing of stress cDNA libraries, 181 ESTs presented putative introns (2.9%), while sequencing of cDNA libraries from unstressed B. emersonii cells revealed only 0.2% of ESTs containing introns. These data indicate an enrichment of ESTs with introns in B. emersonii stress cDNA libraries. Among the 85 genes corresponding to the ESTs that retained introns, 19 showed more than one intron and three showed three introns, with intron length ranging from 55 to 333 nucleotides. Canonical splicing junctions were observed in most of these introns, junction sequences being very similar to those found in introns from genes previously characterized in B. emersonii, suggesting that inhibition of splicing during stress is apparently a random process. Confirming our observations, analyses of gpx3 and hsp70 mRNAs by Northern blot and S1 protection assays revealed a strong inhibition of intron splicing in cells submitted to cadmium stress.ConclusionIn conclusion, data indicate that environmental stresses, particularly cadmium treatment, inhibit intron processing in B. emersonii, revealing a new adaptive response to cellular exposure to this heavy metal.
| S-EPMC2773782 | biostudies-literature
Project description:Global gene expression analysis was carried out with Blastocladiella emersonii cells subjected to oxygen deprivation (hypoxia) using cDNA microarrays. In experiments of gradual hypoxia (gradual decrease in dissolved oxygen) and direct hypoxia (direct decrease in dissolved oxygen), about 650 differentially expressed genes were observed. A total of 534 genes were affected directly or indirectly by oxygen availability, as they showed recovery to normal expression levels or a tendency to recover when cells were reoxygenated. In addition to modulating many genes with no putative assigned function, B. emersonii cells respond to hypoxia by readjusting the expression levels of genes responsible for energy production and consumption. At least transcriptionally, this fungus seems to favor anaerobic metabolism through the upregulation of genes encoding glycolytic enzymes and lactate dehydrogenase and the downregulation of most genes coding for tricarboxylic acid (TCA) cycle enzymes. Furthermore, genes involved in energy-costly processes, like protein synthesis, amino acid biosynthesis, protein folding, and transport, had their expression profiles predominantly downregulated during oxygen deprivation, indicating an energy-saving effort. Data also revealed similarities between the transcriptional profiles of cells under hypoxia and under iron(II) deprivation, suggesting that Fe(2+) ion could have a role in oxygen sensing and/or response to hypoxia in B. emersonii. Additionally, treatment of fungal cells prior to hypoxia with the antibiotic geldanamycin, which negatively affects the stability of mammalian hypoxia transcription factor HIF-1alpha, caused a significant decrease in the levels of certain upregulated hypoxic genes.
| S-EPMC2901646 | biostudies-literature
Project description:We explored transcriptional responses of the chytridiomycete Blastocladiella emersonii to some stress environmental conditions. Three distinct cDNA libraries constructed from mRNAs extracted from cells submitted to heat shock and different cadmium concentrations (50 and 100 mM) were sequenced and generated 6,350 high-quality EST sequences. These ESTs were assembled into 2,326 unigenes, being 51% of them not previously described in B. emersonii and consequently represent a worthy contribution of this work. We assigned to approximately 59% of the unigenes an orthologue in other organism, whereas 41% remained without a putative identification. Using the transcriptome data of B. emersonii we constructed a microarray slide containing 3,773 different cDNAs which comprise many biological processes of this fungus and analyzed its expression pattern in response to cadmium and heat shock. Through cDNA microarray assays we observed that 3,3% and 5,1% of the genes present in B. emersonii microarray slide were up-regulated in response to heat shock and cadmium, respectively. The main categories to which belong these genes were protein folding and proteolysis, proteins with antioxidant properties, cellular transport, carbohydrate and amino acid metabolism. Interestingly, in response to cadmium stress, B. emersonii induced six different genes codifying Glutathione S-transferases and six Metacaspases, as well as several proteins from sulfur amino acid metabolism. Keywords: stress response; heavy metal stress response; heat shock response
2007-06-22 | GSE6231 | GEO