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Bioinformatic analysis of Protein Disulfide Isomerase A1 (PDIA1)-associated pathways towards developing stratified breast cancer therapies

ABSTRACT: Bioinformatic analysis of Protein Disulfide Isomerase A1 (PDIA1)-associated pathways towards developing stratified breast cancer therapies

PROVIDER: PRJNA780603 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16953104.fastq.gz	Fastqsanger.gz
	SRR16953105.fastq.gz	Fastqsanger.gz
	SRR16953106.fastq.gz	Fastqsanger.gz
	SRR16953107.fastq.gz	Fastqsanger.gz
	SRR16953108.fastq.gz	Fastqsanger.gz

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Bioinformatic analysis of Protein Disulfide Isomerase A1 (PDIA1)-associated pathways towards developing stratified breast cancer therapies

Project description:The oxidoreductase protein disulfide isomerase A1 (PDIA1) functions as a cofactor for many transcription factors including the estrogen receptor alpha (ERα), the NF-κΒ, the nuclear factor erythroid 2-like 2 (NRF2) and regulates the protein stability of the tumor suppressor p53. Taking this into account we hypothesized that PDIA1, by differentially modulating the gene expression of diverse subsets of genes in the ERα positive versus the ERα negative breast cancer cells, modifies dissimilar pathways in the two types of breast cancer. This hypothesis was investigated using RNA-seq data from PDIA1-silenced MCF-7 (ERα+) and MDA-MB-231 (ERα-) breast cancer cells treated with either interferon gamma (IFN-γ) or etoposide (ETO) and the obtained data were further analyzed using a variety of bioinformatic tools alongside clinical relevance assessment via Kaplan-Meier patient survival curves. The results highlighted the dual role of PDIA1 in suppressing carcinogenesis in the ERα+ breast cancer patients by negatively regulating the response to reactive oxygen species and promoting carcinogenesis by inducing cell cycle progression. In the ERα- breast cancer patients PDIA1 prevents tumor development by regulating the NF-kappa B and p53 by modulating cell migration and inducing breast cancer progression through control of cytokine signaling and the immune response. The findings reported in this study shed light on the differential pathways regulating carcinogenesis in the ERα+ and ERα- breast cancer patients and could help identify therapeutic targets selectively effective in the ERα+ versus the ERα- patients.

2021-11-18 | GSE188914 | GEO

Dual roles for a tick protein disulfide isomerase during the life cycle of the Lyme disease agent

Project description:To understand the immunomodulation roles of Ixodes scapularis protein disulfide isomerase A6 (IsPDIA6), we utilized RNA sequencing (RNA-seq) to investigate the effect of IsPDIA6 on the murine transcriptome.

2024-10-14 | GSE273486 | GEO

Dual roles for a tick protein disulfide isomerase during the life cycle of the Lyme disease agent [I. scapularis]

Project description:To determine whether the influences of Ixodes scapularis protein disulfide isomerase A6 (IsPDIA6) on Borrelia burgdorferi colonization is the result of physiological responses, we performed RNA-sequencing (RNA-seq) to compare the transcriptomes of control (GFP) and IsPDIA6-silenced ticks.

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Therapeutic Targeting of Protein Disulfide Isomerase PDIA1 in Multiple Myeloma

Project description:Therapeutic Targeting of Protein Disulfide Isomerase PDIA1 in Multiple Myeloma

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Expression data from SH-SY5Y neuroblastoma cells treated with the protein disulfide isomerase inhibitor origamicin

Project description:Protein disulfide isomerase (PDI) is associated with many diseases including neurodegenerative disorders like Alzheimer’s disease and, hence, is considered a promising drug target. In this study, we investigate the abscisic acid (ABA)-derived PDI inhibitor origamicin for its neuropharmacological potential. We used microarrays to analyze the global change in gene expression as a result of PDI inhibition in the human neuroblastoma cell line SH-SY5Y

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Protective Role for the Disulfide Isomerase PDIA3 in Methamphetamine Neurotoxicity

Project description:Methamphetamine abuse continues to be a worldwide problem, damaging the individual user as well as society. Only minimal information exists on molecular changes in the brain that result from methamphetamine administered in patterns typical of human abusers. In order to investigate such changes, we examined the effect of methamphetamine on the transcriptional profile in brains of monkeys. Gene expression profiling of the caudate and hippocampus identified protein disulfide isomerase family member A3 (PDIA3) to be significantly up-regulated in the animals treated with methamphetamine as compared to saline treated control monkeys. Treatment of primary rat neurons with methamphetamine revealed an up-regulation of PDIA3, showing a direct effect of methamphetamine on neurons to increase PDIA3. In vitro studies using a neuroblastoma cell line demonstrated that PDIA3 expression protects against methamphetamine-induced cell toxicity and methamphetamine-induced intracellular reactive oxygen species production, revealing a neuroprotective role for PDIA3. The current study implicates PDIA3 to be an important cellular neuroprotective mechanism against a toxic drug, and as a potential target for therapeutic investigations. To study the effects of chronic METH effects on the brain

2011-11-15 | E-GEOD-33707 | biostudies-arrayexpress

Treatment of Neuroblastoma Cells with Inhibitors of Protein Disulfide Isomerase Upregulates NQO1 Activity

Project description:Protein disulfide isomerase (PDI) is associated with many diseases including neurodegenerative disorders like Alzheimer’s disease and, hence, is considered a promising drug target. This study employs the two PDI inhibitors securinine and 16F16 in order to interrogate the role of PDI in the neurological disease and presents a novel link between PDI inhibition and NQO1 activity Microarrays were used to analyze the global change in gene expression as a result of PDI inhibition in the human neuroblastoma cell line SH-SY5Y

2020-12-31 | GSE153259 | GEO

Identification of transcripts induced after treatment with protein disulfide isomerase inhibitor E64FC65 and panobinostat in PANC-1 cells

Project description:Protein disulfide isomerase and histone deacetylase inhibition induces a synergistic pro-apoptotic response. The goal of this RNA-Seq experiment is to (1) identify critical transcripts and pathways induced by the combination in PANC-1 cells that promote apoptosis and (2) conduct RNA-seq in parallel with ATF3 knockdown to identify ATF3-dependent target genes.

2019-12-18 | GSE142210 | GEO

Protective Role for the Disulfide Isomerase PDIA3 in Methamphetamine Neurotoxicity

2011-11-15 | GSE33707 | GEO

Treatment of Neuroblastoma Cells with Inhibitors of Protein Disulfide Isomerase Upregulates NQO1 Activity

Project description:Treatment of Neuroblastoma Cells with Inhibitors of Protein Disulfide Isomerase Upregulates NQO1 Activity

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