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Rapid Acceleration of Diagnostics - Radical (RADx-rad): Nanobody-based Electrochemical Biosensor for Real-Time Detection of Aerosolized SARS-CoV2

ABSTRACT: Rapid Acceleration of Diagnostics - Radical (RADx-rad): Nanobody-based Electrochemical Biosensor for Real-Time Detection of Aerosolized SARS-CoV2

PROVIDER: PRJNA781720 | ENA |

REPOSITORIES: ENA

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Rapid Acceleration of Diagnostics - Radical (RADx-rad): Nanobody-based Electrochemical Biosensor for Real-Time Detection of Aerosolized SARS-CoV2

Project description:Rapid Acceleration of Diagnostics - Radical (RADx-rad): Nanobody-based Electrochemical Biosensor for Real-Time Detection of Aerosolized SARS-CoV2

| PRJNA781722 | ENA

Construction of Synthetic Nanobody Library in Mammalian Cells by dsDNA-Based Strategies

Project description:A nanobody is an antibody fragment consisting of a single monomeric variable antigen-binding domain. Mammalian cells are ideal platforms for identifying nanobodies targeting hard-to-display transmembrane proteins and nanobodies that function as modulators of cellular phenotypes. However, the introduction of a high-diversity nanobody library into mammalian cells is challenging. We have developed two novel methods for constructing a nanobody library in mammalian cells. Complementarity-determining region (CDR) random sequences were first incorporated into upstream and downstream dsDNAs by PCR. In the first method, named dsDNA-HR, upstream and downstream dsDNAs containing an identical overlapping sequence were co-transfected into cultured mammalian cells for intracellular homologous recombination that resulted in the formation of an intact nanobody library expression cassette. In the second method, named in vitro ligation, we generated full-length nanobody expression dsDNAs via ligation of restriction digested upstream and downstream dsDNAs. The obtained full-length dsDNAs were transfected into mammalian cells for nanobody library expression. Using both methods, we generated over a million unique nanobody sequences, as revealed by high-throughput sequencing. Single-cell sequencing was employed to resolve the diversity of the dsDNA-HR nanobody library. We also identified a small molecule, Nocodazole, which could enhance the efficacy of dsDNA-HR.

2021-09-21 | GSE158906 | GEO

Biparatopic anti-spike nanobody

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Bulk RNA-seq of an ALI (Air-lung interface) model of SARS-CoV2

Project description:This dataset looks at the transcriptome of in vitro-differentiated primary lung cells infected with SARS-CoV2. Some cells have been treated with the drug Enzalutamide.

2022-05-04 | E-MTAB-10687 | biostudies-arrayexpress

PS19 brain lysate transfection changing ER stress gene expression in Biosensor cell

Project description:To investigate whether PS19 brain lysate transfection changes ER stress gene expression in Biosensor cell

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IL-9 exacerbates SARS-CoV2 infection and associated airway inflammation

Project description:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes coronavirus diseases 2019 (COVID-19) and broncho-alveolar inflammation (Merad and Martin, 2020). IL-9 induces airway inflammation and bronchial hyper responsiveness in respiratory viral illnesses and allergic inflammation (Temann et al., 1998). However, the role of IL-9 is not yet identified in SARS-CoV2 infection. Here we show that IL-9 promotes SARS-CoV2 infection and airway inflammation in K18-hACE2 transgenic (ACE2.Tg) mice, as IL-9 blockade reduces SARS-CoV2 infection and suppressed airway inflammation. Foxo1 is essential for the induction of IL-9 in helper T (Th) cells (Malik et al., 2017). While ACE2.Tg mice with Foxo1-deficiency in CD4+ T cells were performed to be resistant to SARS-CoV2 infection associated with reduced IL-9 production, exogenous IL-9 made Foxo1-deficient mice susceptible to SARS-CoV2 infection with increased airway inflammation. Collectively, we identify a mechanistic insight of IL-9-mediated regulation of antiviral and inflammatory pathways in SARS-CoV2 infection, and unravel a principle for the development of host-directed therapeutics to mitigate disease severity.

2022-07-22 | GSE209550 | GEO

Csf2ra deletion attenuates acute lung injuries induced by intratracheal inoculation of aerosolized ricin in mice

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Transcriptomics analysis of SARS-CoV2-induced changes in human lung epithelial Calu-3 cells

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2022-10-25 | E-MTAB-12134 | biostudies-arrayexpress

Mice inflammatory responses to inhaled aerosolized LPS: effects of various forms of human alpha1-antitrypsin

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Online electrochemical reduction of both inter- and intramolecular disulfide bridges in immunoglobulins

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2022-05-27 | PXD021649 | Pride

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