Project description:Lysinibacillus varians GY32 is a filamentous bacteria that can generate electricity in microbial fuel cells. To find potential genes participating in the electron transfer to electrode of Lysinibacillus varians GY32, we compared the gene expression profiles of this bacteria with yeast extract as electron donor and two electron acceptors, i.e. oxygen and electrode in microbial fuel cells. The results showed that several cytochrome c genes might play specific roles in the extracellular electron transfer to electrode in this strain.
Project description:Lysinibacillus varians GY32 is a filamentous bacteria that can generate electricity in microbial fuel cells. To find potential genes participating in the electron transfer to electrode of Lysinibacillus varians GY32, we compared the gene expression profiles of this bacteria with acetate as electron donor and two electron acceptors, i.e. oxygen and electrode in microbial fuel cells. The results showed that several cytochrome c genes might play specific roles in the extracellular electron transfer to electrode in this strain.
Project description:Purpose: this study provided a comprehensive sequence for a systemic view of the transcriptome between mango leaf and fruit, as well as fruit allergens, which will be useful for further genomic research studies and breeding of lower allergenic mango cultivars. Methods:Some allergens have previously been identified in mango (Mangifera indica Linn), including profilins, Bet v 1-like proteins and chitinase. In this paper, 66 potential allergen genes were identified and their relative expressions evaluated in mango fruit and leaf using Illumina RNA-Seq technology. Results:A total of 17.63Gb Clean Data was obtained.The number of %≥Q30 was above 94.58%.RNA-Seq generated 11,751,123 contigs that were assembled into 99,328 unigenes with 16,848 unigenes of >1000 bp. A total of 230,242 unigenes were annotated using public protein databases, with a cut-off E-value above 10−5, of which 27,295, 46,030, 24,227 and 14,023 unigenes were assigned to gene ontology terms, Nr, Swiss-Prot and clusters of orthologous groups, respectively. Allergens mainly belonged to pollen allergen, pathogenesis-related protein Bet v I family and NADPH-dependent FMN reductase.
Project description:RNA-seq based indentification of responsive and critical genes which might be involved in the arsenopyrite (FeAsS) weathering by Lysinibacillus sp. B2A1