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MiRNA data from C8+ T cells exposed to DCMU or control vehicle (DMSO)

ABSTRACT: miRNA data from C8+ T cells exposed to DCMU or control vehicle (DMSO)

PROVIDER: PRJNA783023 | ENA |

REPOSITORIES: ENA

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mRNA data from C8+ T cells exposed to DCMU or control vehicle (DMSO)

Project description:mRNA data from C8+ T cells exposed to DCMU or control vehicle (DMSO)

| PRJNA783017 | ENA

miRNA data from C8+ T cells exposed to DCMU or control vehicle (DMSO)

Project description:DCMU, or diuron, is a widely used herbicide, which can cause adverse effects on human. Here, we develop an approach that combines functional, miRNA and RNA sequencing data to evaluate the effects of DCMU exposure on the human immune system, notably on CD8+ T cells, as the main effectors of the organism’s defense against viruses or neoplastic cells. We found that DCMU reduces T cell abilities, in vitro but also in vivo in orthotopic mouse and zebrafish models, participating to the establishment of a suitable environment for the development of more serious diseases such as cancers. We identified several miRNAs, such as hsa-miR3135b and hsa-miR-21-5p, respectively induced and repressed by DCMU, which alters CD8+ T cell functions. The major advancement of our study is the description of the molecular mechanisms induced by DCMU that control human immune functions.

2022-07-05 | GSE189443 | GEO

mRNA data from C8+ T cells exposed to DCMU or control vehicle (DMSO)

2022-07-05 | GSE189440 | GEO

DNA methylation profiling of cells exposed to DNMT1 inhibitor GSK-3484862 or DMSO (vehicle)

Project description:DNA methylation profiling of cells exposed to DNMT1 inhibitor GSK-3484862 or DMSO (vehicle)

| PRJNA1254134 | ENA

DNA methylation profiling of cells exposed to DNMT1 inhibitor GSK-3484862 or DMSO (vehicle)

Project description:By relating the numbers of oxidized guanines in different sequence contexts to the abundance of these contexts in the human genome, we observed that the probability of guanine to be or remain oxidized is low if this guanine is preceded by cytosine (CpG sites) compared to other nucleotides. As CpG sites are substrates of epigenetic cytosine methylation, we investigated the influence of cytosine methylation status on guanine oxidation level in CpG sites. For this, we exposed HAP1 and U2OS cells for three days to GSK-3484862, a novel DNA methyltransferase 1 (DNMT1) inhibitor causing the degradation of this enzyme within 24 hours and having low cellular toxicity. Using Infinium MethylationEPIC v2.0 array, we observed a drastic hypomethylation after the exposure in both cell lines such that the peak of highly methylated CpG sites (beta values 0.8–1) found in vehicle (DMSO) exposed cells is absent in GSK-3484862-exposed cells. In contrast, the oxidation level of guanines in the CpG sites profiled by the array did not change in response to the inhibitor and was virtually constant across CpG sites with different methylation levels. In addition, on the level of all CpG sites in the genome, guanine-oxidation did not differ between DMSO and GSK-3484862 exposures. Thus, in CpG sites, guanine oxidation level likely does not depend on cytosine methylation status.

2025-08-22 | GSE295295 | GEO

Enhanced RNA Interactome capture (eRIC) and RNA Interactome capture (RIC) of Jurkat cells (DSMZ, ACC-282) exposed to 0.5mM DMOG or vehicle (DMSO) for 6 hours.

Project description:1.0 â€“ 1.3 x 10e8 proliferating Jurkat cells at a density of about 1-1.5 x 10e6 cells/mL were employed per sample. Cells were incubated with 0.5mM DMOG (Cayman Chemical Company, 71210) or an equivalent volume of Dimethyl Sulfoxide (DMSO) (vehicle, Merck 1.02950.0500). The DMSO concentration in the medium was 0.023% v/v. Cells were irradiated or not with 254 nm UV light and subjected to eRIC or RIC to determine the RNA-bound proteome. Data correspond to two biologically independent experiments.

2018-09-03 | PXD010942 | Pride

LX-2 cells pretreated with CM272 (400nM) or vehicle (0.01%DMSO)

Project description:LX-2 cells were pretreated with CM272 (400nM) or vehicle (0.01%DMSO) for 24 hours followed by incubation of TGFβ1 (5ng/ml) for another 24 hours.

2019-10-29 | GSE139504 | GEO

Microarray data from BM-MSCs exposed to DMSO, LG268, Rosi, TPhP, TBT, and MEHP

Project description:Primary mouse bone marrow mesenchymal stromal cells (BM-MSCs) cultured for bone differentiation were exposed to vehicle (DMSO), synthetic RXR ligand (LG100268), type 2 diabetes therapeutic and PPARγ ligand (Rosiglitazone), and environmental PPARγ ligands (Tributyltin, Triphenyl Phosphate, and MEHP) to evaluate differential gene expression as well as nuclear receptor expression and downstream PPARG target gene expression between all chemical exposures

2018-09-06 | GSE119541 | GEO

Vibrio sp. C8 strain:C8

Project description:Vibrio sp. C8 strain:C8 Genome sequencing

| PRJNA1271246 | ENA

FOXO-inhibited iPSC-derived cardiomyocytes compared against DMSO vehicle controls.

Project description:Human induced pluripotent stem cell (iPSC)-derived cardiomyocytes cultured in 3-dimensional suspension culture were treated with AS1842856 FOXO-inhibitor (n=3 independent wells) or DMSO vehicle control (n=3 independent wells). Total RNA was isolated from each well and utilized for RNA sequencing.

2024-08-09 | GSE252627 | GEO

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