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Function analysis and characterisation of a novel chitinase, MdCht9, in Musca domestica

ABSTRACT: Function analysis and characterisation of a novel chitinase, MdCht9, in Musca domestica

PROVIDER: PRJNA783282 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17027328_1.fastq.gz	Fastqsanger.gz
	SRR17027328_2.fastq.gz	Fastqsanger.gz
	SRR17027329_1.fastq.gz	Fastqsanger.gz
	SRR17027329_2.fastq.gz	Fastqsanger.gz
	SRR17027330_1.fastq.gz	Fastqsanger.gz

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Function analysis and characterisation of a novel chitinase, MdCht9, in Musca domestica

Project description:To investigate how MdCht9 contribute to the development and digestion of Musca domestica, we silenced the chitinase gene 9 (MdCht9) by its dsRNA, while silenced the GFP as the control. Then we compared the differental expression genes between the test group and the control group.

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Project description:Illumina HiSeq 2000 RNASeq was conducted on the mRNA fraction of three adult female Musca domestica strains. Two strains were the insecticide susceptible strains CS and aabys, which has five distinct recessive morphological markers, one on each of the five autosomes in M. domestica. The third strain was ALHF, which is a highly insecticide resistant strain obtained in Alabama. The reference transcriptome was generated de novo using Trinity. The coding trimmed sequences from Augustus were then used as the mapping reference for the individual FASTQ files from each strain. trace reads were mapped using RSEM to generate FPKM estimates. Gene expression levels were compared to the ALHF strain using EdgeR to identify genes that were downregulated in both aabys and CS. RNA Seq profiling of the mRNA of adult Musca domestica with different autosomal contributions from insecticide resistant and susceptible strains (whole insect pooled samples)

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