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Animal faeces and wastewater samples isolated from a pig farm.

ABSTRACT: Animal faeces and wastewater samples isolated from pig farms. Raw sequence reads

PROVIDER: PRJNA783993 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17188500_1.fastq.gz	Fastqsanger.gz
	SRR17188500_2.fastq.gz	Fastqsanger.gz
	SRR17188501_1.fastq.gz	Fastqsanger.gz
	SRR17188501_2.fastq.gz	Fastqsanger.gz
	SRR17188502_1.fastq.gz	Fastqsanger.gz

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Multiresistant Bacteria Isolated from Intestinal Faeces of Farm Animals in Austria.

Project description:In recent years, antibiotic-resistant bacteria with an impact on human health, such as extended spectrum β-lactamase (ESBL)-containing Enterobacteriaceae, methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococci (VRE), have become more common in food. This is due to the use of antibiotics in animal husbandry, which leads to the promotion of antibiotic resistance and thus also makes food a source of such resistant bacteria. Most studies dealing with this issue usually focus on the animals or processed food products to examine the antibiotic resistant bacteria. This study investigated the intestine as another main habitat besides the skin for multiresistant bacteria. For this purpose, faeces samples were taken directly from the intestines of swine (n = 71) and broiler (n = 100) during the slaughter process and analysed. All samples were from animals fed in Austria and slaughtered in Austrian slaughterhouses for food production. The samples were examined for the presence of ESBL-producing Enterobacteriaceae, MRSA, MRCoNS and VRE. The resistance genes of the isolated bacteria were detected and sequenced by PCR. Phenotypic ESBL-producing Escherichia coli could be isolated in 10% of broiler casings (10 out of 100) and 43.6% of swine casings (31 out of 71). In line with previous studies, the results of this study showed that CTX-M-1 was the dominant ESBL produced by E. coli from swine (n = 25, 83.3%) and SHV-12 from broilers (n = 13, 81.3%). Overall, the frequency of positive samples with multidrug-resistant bacteria was lower than in most comparable studies focusing on meat products.

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