Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Halo-enhanced Ago2 pulldown (HEAP) to identify miRNA targets in murine colonic tumor +/- oncogenic K-Ras-G12D

ABSTRACT: Halo-enhanced Ago2 pulldown (HEAP) to identify miRNA targets in murine colonic tumor +/- oncogenic K-Ras-G12D

PROVIDER: PRJNA784253 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR17055000.fastq.gz	Fastqsanger.gz
	SRR17055001.fastq.gz	Fastqsanger.gz
	SRR17055002.fastq.gz	Fastqsanger.gz
	SRR17055003.fastq.gz	Fastqsanger.gz
	SRR17055004.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 144

Similar Datasets

Halo-enhanced Ago2 pulldown (HEAP) to identify miRNA targets in vivo

Project description:The identification of miRNA targets by Ago2-CLIP methods has provided major insights into the biology of this important class of non-coding RNAs. However, these methods are technically challenging and not easily translated to an in vivo setting. To overcome these limitations and to facilitate the investigation of miRNA functions in mice, we have developed a method (HEAP) to map miRNA-mRNA binding sites. This method uses a novel mouse strain harboring a conditional Halo-Ago2 allele expressed from the Ago2 locus. By using a resin conjugated to the HaloTag ligand, Ago2-miRNA-mRNA complexes can be efficiently purified from cells and tissues expressing Halo-Ago2. We demonstrate the reproducibility and sensitivity of this method in embryonic stem cells, in embryos, in adult tissues and in autochthonous mouse models of cancers. These tools and datasets are valuable resources to the scientific community and will facilitate the characterization of miRNA functions under physiological and pathological conditions.

2019-10-27 | GSE139344 | GEO

Halo-enhanced Ago2 pulldown (HEAP) to identify miRNA targets in vivo

Project description:Halo-enhanced Ago2 pulldown (HEAP) to identify miRNA targets in vivo

| PRJNA579359 | ENA

Halo-enhanced Ago2 pulldown (HEAP) to identify miRNA targets in murine colonic epithelium +/- oncogenic K-Ras-G12D

Project description:Halo-enhanced Ago2 pulldown (HEAP) to identify miRNA targets in murine colonic epithelium +/- oncogenic K-Ras-G12D

| PRJNA784254 | ENA

Halo-enhanced Ago2 pulldown (HEAP) to identify miRNA targets in mouse embryonic stem cells (mESCs)

2019-10-27 | GSE139345 | GEO

Halo-enhanced Ago2 pulldown (HEAP) to identify miRNA targets in mouse embryonic stem cells (mESCs)

Project description:Halo-enhanced Ago2 pulldown (HEAP) to identify miRNA targets in mouse embryonic stem cells (mESCs)

| PRJNA579358 | ENA

Halo-enhanced Ago2 pulldown (HEAP) to identify miRNA targets in murine colonic tumor +/- oncogenic K-Ras-G12D

Project description:K-Ras is frequently hyperactivated in human cancers through gain-of-function mutations that drive tumorigenesis. K-RasG12D, the most common oncogenic K-Ras allele, triggers massive transcriptomic and proteomic changes in the murine colon. Here, we report a comprehensive profile of physiological miRNA targets in murine colonic epithelium and tumor expressing K-RasG12D. Combining it with transcriptional, transcriptomic, and proteomic landscapes, we uncover a K-RasG12D-induced global suppression of miRNA activity that up-regulates hundreds of genes post-transcriptionally. K-RasG12D suppresses Csnk1a1 and Csnk2a1, which can decrease Ago2 phosphorylation at Ser825/829/831/835. Hypo-phosphorylated Ago2 increases binding with mRNA, reducing its regulatory activity by locking Ago2 in a small set of target transcripts. While expanding the repertoire of miRNA targets identified, it functionally decreases active Ago2, resulting in global de-repression of miRNA targets. Our findings establish a regulatory relationship among K-Ras, Csnk1a1/Csnk2a1, and Ago2 that provides a mechanistic link between oncogenic K-Ras and the up-regulation of hundreds of miRNA targets.

2022-05-13 | GSE189701 | GEO

Halo-enhanced Ago2 pulldown (HEAP) to identify miRNA targets in murine colonic epithelium +/- oncogenic K-Ras-G12D

2022-05-13 | GSE189700 | GEO

Efficient in vivo mapping of miRNA targets by Halo-enhanced Ago2 pulldown

Project description:Efficient in vivo mapping of miRNA targets by Halo-enhanced Ago2 pulldown

| PRJNA579349 | ENA

Comparing two approaches of miR-34a target identification, biotinylated-miRNA pulldown vs miRNA overexpression

Project description:Here we show that biotin-labelled miR-34a can be loaded to AGO2, and AGO2 immunoprecipitation can pulldown biotinylated miR-34a (Bio-miR pulldown). RNA-sequencing (RNA-seq) of the Bio-miR pulldown RNAs efficiently identified miR-34a mRNA targets, which could be verified with luciferase assays. In contrast to the approach of Bio-miR pulldown, RNA-seq of miR-34a overexpression samples had limited value in identifying direct targets of miR-34a. It seems that pulldown of 30 -Biotin-tagged miRNA can identify bona fide microRNA targets at least for miR34a.

2019-02-27 | GSE127211 | GEO

Ribo-depleted RNA-seq in KRasG12D; p53-null (KP) lung adenocarcinoma cells to measure gene expression changes upon the expression of a T6B-YFP fusion

2019-10-27 | GSE139346 | GEO