Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNAseq analysis of CD4+EGFP+ and CD8+EGFP+ regulatory T cells

ABSTRACT: RNAseq analysis of CD4+EGFP+ and CD8+EGFP+ regulatory T cells

PROVIDER: PRJNA784567 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR17066200.fastq.gz	Fastqsanger.gz
	SRR17066201.fastq.gz	Fastqsanger.gz
	SRR17066202.fastq.gz	Fastqsanger.gz
	SRR17066203.fastq.gz	Fastqsanger.gz
	SRR17066204.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 49

Similar Datasets

RNAseq analysis of CD4+EGFP+ and CD8+EGFP+ regulatory T cells

Project description:Gene-edited rats were generated in which EGFP was placed under the transcriptional control of the Foxp3 promoter. EGFP expression by CD4+ and CD8+ T cells allowed to define regulatory T cells (Treg) in both T cell compartments. This Foxp3-EGFP rats constitute a useful model to identify CD4+ and CD8+ natural and induced Treg. Transcriptomic analyses showed similarities but also differences among CD4+ and CD8+ EGFP+ cells, this being the first description of the transcriptomic profile in natural FOXP3+ CD8+ Treg.

2022-11-02 | GSE189797 | GEO

Expression Profile of alloreactive CD8 and CD4 induced regulatory T cells

Project description:Adoptive natural regulatory T cell (nTreg) therapy has improved the outcome for patients suffering from graft-versus-host disease (GVHD) following allogeneic hematopoietic cell transplantation (allo-HCT). However, fear of broad immune suppression and subsequent dampening of beneficial graft-versus-leukemic (GVL) responses remains a challenge. To address this concern, we generated alloreactive induced Tregs (iTregs) from resting CD4 or CD8 T cells and tested their ability to suppress GVH and maintain GVL responses. We utilized major mismatched and haploidentical murine models of HCT with host-derived lymphoma or leukemia cell lines to evaluate GVH and GVL responses simultaneously. Alloreactive CD4 iTregs were effective in preventing GVHD, but abrogated the GVL effect against aggressive leukemia. Alloreactive CD8 iTregs moderately attenuated GVHD while sparing the GVL effect. Hence, we reasoned that using a combination of CD4 and CD8 iTregs could achieve the optimal goal of allo-HCT. Indeed, the combinational therapy was superior to CD4 or CD8 iTreg singular therapy in GVHD control; importantly, the combinational therapy maintained GVL responses. Cellular analysis uncovered potent suppression of both CD4 and CD8 effector T cells by the combinational therapy that resulted in effective prevention of GVHD, which could not be achieved by either singular therapy. Gene expression profiles revealed alloreactive CD8 iTregs possess elevated expression of multiple cytolytic molecules compared to CD4 iTregs, which likely contributes to GVL preservation. Our study uncovers unique differences between alloreactive CD4 and CD8 iTregs that can be harnessed to create an optimal iTreg therapy for GVHD prevention with maintained GVL responses.

2016-11-17 | GSE75118 | GEO

RNAseq analysis of mouse CD4+ regulatory Tregs in WT and ∆Foxp3 mice isolated from spleen and lungs.

Project description:RNAseq analysis of mouse CD4+ regulatory Tregs in 21-day-old male WT and ∆Foxp3 mice isolated from spleen and lungs.

2021-02-18 | GSE166860 | GEO

RNAseq analysis of human CD4+ regulatory Tregs and Tconvs in IPEX and healthy donors isolated from peripheral blood.

Project description:RNAseq analysis of human CD4+ regulatory Tregs and Tconvs in IPEX and healthy donors isolated from peripheral blood.

2021-02-18 | GSE166866 | GEO

Comparison of IL10-eGFP+ cells with IL10-eGFP- CD4+ T cells induced by in vivo immunisation

Project description:Here we perform QuantSeq 3' mRNA sequencing of RNA extracted from flow sorted splenic CD4+ Il10-eGFP+ or CD4+Il10eGFP- T cells from Tg4 Nr4a3-Tocky Il10-eGFP mice 24 hours after immunisation with 4 mg/kg of [4Y]-MBP. Our aim was to confirm the Tr1 cell phenotype within the Il10-eGFP+ T cell subset

2024-12-09 | GSE283286 | GEO

Bulk RNAseq analysis of CD4 CD8 double negative 3 (DN3) and CD4 CD8 double positive (DP) thymocytes in thymus autonomy and turnover

Project description:Bulk RNAseq analysis of CD4 CD8 double negative 3 (DN3) and CD4 CD8 double positive (DP) thymocytes in thymus autonomy and turnover

| PRJNA668067 | ENA

RNAseq analysis of human CD4+ regulatory Tregs and Tconvs in COVID-19 and healthy donors isolated from peripheral blood.

Project description:RNAseq analysis of human CD4+ regulatory Tregs and Tconvs in COVID-19 and healthy donors isolated from peripheral blood.

2021-07-07 | GSE179448 | GEO

RNAseq analysis of CD4 and CD8 T cells in response to vaccinia virus infection

Project description:Transcription profiling of mouse CD4+ and CD8+ T cells extracted from GFP-Egr2 knockin (Egr2 Kin) and hCD2-Cre / Egr2loxP/loxP / Egr3-/- Egr2/3 DKO) mice 7 days after infection with vaccinia virus.

2017-06-30 | E-MTAB-5338 | biostudies-arrayexpress

Gene expression of CD4+CD8+ Tfh cells and CD4+CD8- Tfh in tonsil.

Project description:Gene expression of CD4+CD8+ Tfh cells and CD4+CD8- Tfh in tonsil.

| PRJNA836786 | ENA

Comparison of IL10-eGFP+ cells with IL10-eGFP- CD4+ T cells induced by in vivo immunisation

Project description:Comparison of IL10-eGFP+ cells with IL10-eGFP- CD4+ T cells induced by in vivo immunisation

| PRJNA1193044 | ENA