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Bromodomain member BRD4 is required for hematopoietic stem cell functions (CUT&RUN)

ABSTRACT: Bromodomain member BRD4 is required for hematopoietic stem cell functions (CUT&RUN)

PROVIDER: PRJNA784623 | ENA |

REPOSITORIES: ENA

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Bromodomain member BRD4 is required for hematopoietic stem cell functions (CUT&RUN)

Project description:To investigate the role of BRD4 in normal hematopoiesis, Brd4 conditional KO mouse model was generated. We purified the Lin-cKit+ cells from bone marrow of WT and Brd4 KO mice and performed the CUT&RUN to study the histone modification and Brd4 occupancy around genome in HSC/HPCs population.

2023-08-20 | GSE189833 | GEO

Bromodomain member BRD4 is required for hematopoietic stem cell functions

Project description:Bromodomain member BRD4 is required for hematopoietic stem cell functions

| PRJNA784616 | ENA

Bromodomain member BRD4 is required for hematopoietic stem cell functions (RNA-Seq)

Project description:Bromodomain member BRD4 is required for hematopoietic stem cell functions (RNA-Seq)

| PRJNA784622 | ENA

Bromodomain member BRD4 is required for hematopoietic stem cell functions (scRNA-Seq)

Project description:Bromodomain member BRD4 is required for hematopoietic stem cell functions (scRNA-Seq)

| PRJNA784621 | ENA

Bromodomain member BRD4 is required for hematopoietic stem cell functions (ATAC-Seq)

Project description:Bromodomain member BRD4 is required for hematopoietic stem cell functions (ATAC-Seq)

| PRJNA784627 | ENA

The bromodomain protein BRD4 regulates splicing during heat shock

Project description:During heat stress cyto-protective genes including heat shock proteins are transcriptionally up-regulated and post-transcriptional splicing is inhibited. In contrast, co-transcriptional mRNA-splicing is maintained. These factors closely resemble the proteotoxic stress response during tumor development. The bromodomain protein BRD4 has been identified as an integral member of the oxidative stress as well as of the inflammatory response. Furthermore, there is evidence for BRD4's role in splicing regulation; Using RNA-Seq analyses we indeed found a significant increase in splicing inhibition, in particular intron retentions, during heat treatment in BRD4-deficient cells, but not under normal conditions. Subsequent experiments revealed that heat stress leads to the recruitment of BRD4 to nuclear stress bodies, to the interaction with the heat shock factor 1 (HSF1) and to the transcriptional up-regulation of non-coding Sat III RNA transcripts. These findings implicate BRD4 as a central regulator of splicing during heat stress. Since BRD4 is a potent target for anti-cancer therapies, our data linking BRD4 to the splicing machinery and the heat stress response - give additional insight into the mode of action of BRD4 inhibitors. WI38 cells have been treated by heatshock and anti BRD4 siRNA and combination.

2016-09-07 | E-GEOD-73471 | biostudies-arrayexpress

Opposing functions of BRD4 isoforms in breast cancer

Project description:Bromodomain-containing protein 4 (BRD4) is a cancer therapeutic target in many ongoing clinical trials disrupting primarily BRD4-regulated transcription programs. A critical role of BRD4 in cancer development has been reported and attributed mainly to the abundant long isoform (BRD4-L). Here we show, by isoform-specific knockdown and endogenous protein detection along with transgene expression, the less abundant BRD4 short isoform (BRD4-S) is oncogenic while BRD4-L is tumor-suppressive in breast cancer cell proliferation and migration as well as mammary tumor formation and metastasis. Through integrated RNA-seq transcriptome and genome-wide ChIP-seq and CUT&RUN association profiling, we identify Engrailed-1 (EN1) homeobox transcription factor as a key BRD4-S coregulator particularly in triple-negative breast cancer. BRD4-S and EN1 co-modulate the extracellular matrix (ECM)-associated matrisome network, including type II cystatin gene cluster, mucin 5 and cathepsin loci, via enhancer regulation of cancer-associated genes and pathways. Our work highlights the importance of targeted therapy for the oncogenic but not tumor-suppressive activity of BRD4.

2020-05-22 | GSE136151 | GEO

Bromodomain member BRD4 is required for hematopoietic stem cell functions (RNA-Seq)

Project description:To investigate the role of BRD4 in normal hematopoiesis, Brd4 conditional KO mouse model was generated. We purified the Lin-cKit+ cells from bone marrow of WT and Brd4 KO mice and performed the RNA-seq to study the molecular changes in each cell population.

2023-08-20 | GSE189834 | GEO

Bromodomain member BRD4 is required for hematopoietic stem cell functions (ATAC-Seq)

Project description:To investigate the role of BRD4 in normal hematopoiesis, Brd4 conditional KO mouse model was generated. We purified the Lin-cKit+ cells from bone marrow of WT and Brd4 KO mice and performed the ATACseq to study the chromatin accessibility in each cell population.

2023-08-20 | GSE189832 | GEO

Bromodomain member BRD4 is required for hematopoietic stem cell functions (scRNA-Seq)

Project description:To investigate the role of BRD4 in normal hematopoiesis, Brd4 conditional KO mouse model was generated. We purified the cKit+ and LSK cells from bone marrow of WT and Brd4 KO mice and performed the single cell RNA-seq to study the molecular changes in each cell population.

2023-08-20 | GSE189835 | GEO

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