Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The bone marrow niche regulates redox and energy balance in leukemia stem cells

ABSTRACT: The bone marrow niche regulates redox and energy balance in leukemia stem cells

PROVIDER: PRJNA785145 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR17084900.fastq.gz	Fastqsanger.gz
	SRR17084901.fastq.gz	Fastqsanger.gz
	SRR17084902.fastq.gz	Fastqsanger.gz
	SRR17084903.fastq.gz	Fastqsanger.gz
	SRR17084904.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

The bone marrow niche regulates redox and energy balance in leukemia stem cells

Project description:The Cxcr4-Cxcl12 axis has been postulated as a critical pathway dictating leukemia stem cell (LSCs) chemoresistance in AML due to its role in controlling cellular egress from the marrow. Nevertheless, the cellular source of Cxcl12 in the AML microenvironment and the mechanism by which Cxcl12 exert its protective role in AML in vivo remain unresolved. We have evaluated the functional role of Cxcl12 secreted by early mesenchymal stromal cells (MSCs) and osteolineage committed cells in acute myeloid leukemia (AML) maintenance in vivo. Our results demonstrate that early MSCs, in contrast to committed osteoblasts, are integral part of the MLL::AF9 derived AML niche and control LSCs maintenance through Cxcl12 secretion. Cxcl12 from MSCs regulates the oxidative state of LSCs and promotes energy metabolism. Furthermore, the protective role of the niche through the activation of the CXCL12-CXCR4 axis, may also represent a biological hallmark in human pediatric and adult AML, hence, reinforcing the notion that targeting the MSCs-derived CXCL12 may help eradicate leukemia.

2022-11-01 | GSE189928 | GEO

A niche-dependent redox rheostat regulates epithelial stem cell fate in the distal colon

Project description:The niche environment surrounding intestinal stem cells (ISCs) varies along the length of intestine and provides key cues that regulate stem cell fate. Here, we investigated the role of cellular redox balance in colonic ISC function. We show that hypoxia and Wnt signaling synergize to restrict the reactive oxygen species (ROS) generating enzyme NADPH oxidase 1 (NOX1) to the crypt base in the distal colon. NOX1 function maintains a more oxidative cell state that licenses cell cycle entry, altering the balance of asymmetric stem cell self-renewal and directing lineage commitment. Mechanistically, cell redox state directs a self-reinforcing circuit that connects hypoxia inducible factor 1 (HIF1a)-dependent signaling with regulation of the metabolic enzyme isocitrate dehydrogenase 1 (IDH1). Our studies show that cellular redox balance is a central and niche-dependent regulator of epithelial homeostasis and regeneration and provide a basis for understanding disease propensity in the distal large intestine.

2025-02-17 | GSE289209 | GEO

A niche-dependent redox rheostat regulates epithelial stem cell fate in the distal colon II

2025-02-17 | GSE289397 | GEO

Bone marrow niche

Project description:Bone marrow niche

| PRJNA429031 | ENA

Gene expression atlas of energy balance brain regions

Project description:Gene expression atlas of energy balance brain regions

| PRJNA738366 | ENA

Bovine granulosa cells of dominant follicles at 60 days postpartum; effect of negative energy balance on transcriptome

Project description:Granulosa cells of dominant follicles originating from dairy cows with severe negative energy balance (BHBH) or mild negative energy balance (BHBL) were compared. Mild negative energy balance (BHBL) is the reference. Two conditions experiment (BHBH and BHBL); Four pools of 3 biological replicates for each group (total = 12 cows for each group); Two technical replicates per pool (dye-swap).

2015-07-31 | E-GEOD-66116 | biostudies-arrayexpress

Gene expression atlas of energy balance brain regions

Project description:This project involved bulk-RNAseq analysis of mouse brain regions involved in energy balance. Two mouse embryonic stem cell controls were included

2021-06-17 | GSE178290 | GEO

NTRC controls chloroplast redox homeostasis through the regulation of the redox balance of 2-Cys Prxs in Arabidopsis

Project description:Thiol-dependent redox regulation is essential for the rapid adaptation of chloroplast metabolism to unpredictable changes of light intensity. The disulfide reductase activity of thioredoxins (Trxs), which relies on photo-reduced ferredoxin (Fdx) and a Fdx-dependent Trx reductase (FTR), constitutes the Fdx-FTR-Trxs system, which links chloroplast redox regulation to light. In addition, chloroplasts harbor an NADPH-dependent Trx reductase (NTR) with a joint Trx domain, NTRC. The activity of these two redox systems is integrated by the balance of the hydrogen peroxide scavenging enzyme 2-Cys peroxiredoxin (2-Cys Prx), which thus plays a key role in maintaining the reducing capacity of chloroplast Trxs in response to light intensity. Based on the severe phenotype of mutant lines lacking NTRC, it is clear that this enzyme plays an essential role in chloroplast redox homeostasis. However, whether the function of NTRC depends on its capacity of reduce 2-Cys Prxs or has additional targets remains unknown. Here, we have addressed this issue by a comparative analysis of the triple mutant of Arabidopsis thaliana, ntrc-2cpab, simultaneously lacking 2-Cys Prxs and NTRC, and the double mutant 2cpab lacking 2-Cys Prxs. The phenotype of the ntrc-2cpab mutant is indistinguishable of the 2cpab mutant, as shown by growth rate, photosynthesis performance, light-dependent redox regulation of enzyme activity and comparative transcriptomics based RNA-Seq. Based on these results, we propose that the function of NTRC in chloroplast redox homeostasis is exerted by the regulation of the redox balance of 2-Cys Prxs rather than by the direct reduction of additional targets.

2021-09-09 | GSE147793 | GEO

Bovine granulosa cells of dominant follicles at 60 days postpartum; effect of negative energy balance on transcriptome

2015-07-31 | GSE66116 | GEO

Energy Balance Drives Diurnal and Nocturnal Brain Transcriptome Rhythms

Project description:Energy Balance Drives Diurnal and Nocturnal Brain Transcriptome Rhythms

| PRJNA952252 | ENA