Project description:The follicular microenvironment, including intra-follicular granulosa cells (GCs),are responsible for the dynamics of ovarian function. However the cellular component of follicular microenvironment as well as the different functional clusters of GCs and the other type cells in preovulatory follicle have not been extensively explored. Here we surveyed the single-cell transcriptome of follicular microenvironment around MII oocytes in six human preovulatory follicles in in-vitro fertilization. There were 6 different cell types in preovulatory follicles, including GCs, epithelial cells, and various immune cells.We identified 9 different functional clusters with different functional transcriptomic profiles in GCs and 5 different functional clusters with different functional transcriptomic profiles in macropahge.Our results provide a comprehensive understanding of the transcriptomic landscape of the preovulatory follicular microenvironment at the single-cell level.
Project description:Cashmere, also known as soft gold, is produced from secondary hair follicles in Cashmere goats and it’s therefore of significance to investigate the molecular profiles during Cashmere goat hair follicle development. However, our current understanding of the machinery underlying Cashmere goat hair follicle remains largely unexplored and researches regarding hair follicle development mainly used the mouse as a research model. To provides comprehensively understanding on the cellular heterogeneity and cell lineage cell fate decisions, we performed single-cell RNA sequencing on 19,705 single cells from induction (embryonic day 60), organogenesis (embryonic day 90) and cytodifferentiation (embryonic day 120) stage fetus Cashmere goat dorsal skin. Unsupervised clustering analysis identified 16 cell clusters and their corresponding cell types were also successfully characterized. Based on cell lineage inference, we revealed detailed transcriptional gene expression profiles during dermal and epidermal lineage cell fate decisions. These works together delineate unparalleled molecular profiles of different cell populations during Cashmere goat hair follicle morphogenesis and provide a valuable resource for identifying biomarkers during Cashmere goat hair follicle development.
Project description:establish mouse ageing single follicle proteome landscape using ultrasensitive proteomics method. There are 9 samples in this datasheets, 2months, 12months and 22months old mouse antral follicle,each sample have 3 replicates..