Ontology highlight
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PROVIDER: PRJNA785882 | ENA |
REPOSITORIES: ENA
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Project description:Diverse lifestyles and evolution strategies of Bipolaris sorokiniana (Sacc.) Shoemaker: spot blotch pathogen of wheat
| PRJNA579095 | ENA
Project description:Diverse lifestyles and evolution strategies of Bipolaris sorokiniana (Sacc.) Shoemaker: spot blotch pathogen of wheat
| PRJNA579091 | ENA
Project description:We addressed the question how the interaction between the beneficial root endophyte Serendipita vermifera (Sv) and the pathogen Bipolaris sorokiniana (Bs) affects fungal behavior and determines barley host responses using a gnotobiotic natural soil-based split-root system for phenotypic and transcriptional analyses.
2019-07-06 | GSE130517 | GEO
Project description:Bipolaris sorokiniana strain:TPQ3 | cultivar:Wheat Genome sequencing
| PRJNA1369740 | ENA
Project description:Transcriptional changes were monitored in the wheat cultivar Renan 24 hours post i noculation with adapted and non-adapted Magnaporthe isolates using the Affymetrix wheat genome array GeneChip®. Wheat plants cv. Renan were grown in a peat and sand (1:1) mix at 23 C in a Sanyo Fitotron growth cabinet (Sanyo Gallenkamp PLC, Loughborough, U.K.) with a 16/8 h, light/dark cycle. Three Magnaporthe isolates were used in this expt, two wheat-adapted isolates (BR32, BR37) and one wheat non-adapted isolate (BR29). Magnaporthe isolates were grown for eleven days on Complete Media Agar at 25 C under a 16/8h, light/dark cycle. Conidia were harvested by flooding the plates with 5 mL of sterile inoculation solution [0.25% (w/v) gelatine and 0.01% (v/v) Tween 20] and scraping the conidia from the surface using a sterile glass rod. Conidia were filtered through sterile miracloth and the density adjusted to 1 x 10 5 conidia mL-1 with inoculation solution. Fourteen day old wheat seedlings mist inoculated with 4 mL of a Magnaporthe conidia suspension and plants were sealed in plastic propagators to maintain relative humidity c.100% and kept at 25 C in the dark for the first 24 hours post inoculation (hpi). Inoculation solution without Magnaporthe conidia was used as a mock-inoculation control. Leaf samples were collected 24 hpi for transcriptomics analysis from three independent biological experiments. Leaf tissue was ground under liquid nitrogen and total RNA extracted using a QIAquick RNeasy Plant Extraction Kit (Qiagen, Hilden, Germany), followed by TURBO DNaseTM (Ambion, Texas, U.S.A.) treatment. RNeasy Mini Spin column purification (Qiagen) was used to further purify RNA samples for array hybridisation. RNA quality checks, cRNA conversion and Affymetrix genome array hybridisation was carried out by the Nottingham Arabidopsis Stock Centre (NASC) array hybridisation service (http://affymetrix.arabidopsis.info/). ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Graham McGrann. The equivalent experiment is TA24 at PLEXdb.] pathogen isolates: Mock-inoculated (Control)(3-replications); pathogen isolates: Wheat non-adapted Magnaporthe isolate BR29(3-replications); pathogen isolates: Wheat adapted Magnaporthe isolate BR32(3-replications); pathogen isolates: Wheat adapted Magnaporthe isolate BR37(3-replications)
2011-08-30 | E-GEOD-31760 | biostudies-arrayexpress
Project description:Candida tropicalis is a leading cause of invasive candidiasis in the Asia-Pacific region with reported crude mortality rates exceeding 50%. The rising prevalence of azole-resistant strains presents a significant clinical challenge. We analyzed 1,016 C. tropicalis clinical isolates collected over nine years from 27 hospitals across North India. Fluconazole resistance was detected in 5.1% (n = 52) of isolates, with cross-resistance observed to voriconazole in 55.7% and itraconazole in 44.2% of isolates. Multilocus sequence typing (MLST) analysis of global 1,630 isolates including 208 Indian and whole-genome sequencing of 716 global isolates (139 Indian) confirmed the clonal emergence and persistence of azole-resistant MLST clade 4 strains in Indian hospitals. Phylogenomic analyses identified that Indian azole-resistant lineage was closely related to azole-resistant isolates from mainland China and Taiwan. The underlying mechanism of resistance involved hotspot mutations (Y132F) in the ERG11 gene along with its duplication, overexpression, and twofold high ergosterol content. Comparative transcriptomics of two clinical isolates exhibiting >512 fold difference in fluconazole susceptibility identified upregulation of virulence-associated genes, ALS7 gene (eightfold), SAP7 and SAP9 (1.6- and 2-fold, respectively) in azole-resistant isolate. Furthermore, azole-resistant isolates showed robust biofilm-associated metabolic activity (twofold), reduced β-glucan exposure, and greater survival in both neutrophil and macrophage killing assays. Notably, azole-resistant lineage exhibits several traits associated with adhesion and immune evasion that could possibly enable its spread in healthcare settings and signals the beginning of a greater spread of this clone. The urgent need for continuous genomic surveillance and antifungal stewardship is warranted to mitigate the spread of multidrug-resistant C. tropicalis.
2026-02-23 | GSE309448 | GEO
Project description:Southern corn leaf blight (SCLB), caused by Bipolaris maydis, poses a serious threat to maize production worldwide. In our previous study, Bacillus velezensis BV3 was isolated and demonstrated strong antagonistic activity against maize leaf spot pathogens and effective disease control in greenhouse experiments.
2026-04-20 | GSE328576 | GEO
Project description:Hordeum vulgare ssp. spontaneum, accession Shechem 12-32, was submitted to 4 experimental treatments (C. sativus (spot blotch), P. hordei (leaf rust), and water and oil controls) to examine gene transcription differences triggered by biotrophic and hemi-biotrophic pathogens. Inoculated plants were arranged in a split plot design. Samples were collected at 12, 24, 36, & 48 hours after inoculation. A total of 48 samples (4 treatments x 4 time points x 3 replicates) were subjected to GeneChip analysis. Made public: 2009-12-02 ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Ben Millett. The equivalent experiment is BB61 at PLEXdb.] treated or untreated: C. sativus (spot blotch) - time: 12 hrs(3-replications); treated or untreated: C. sativus (spot blotch) - time: 24 hrs(3-replications); treated or untreated: C. sativus (spot blotch) - time: 36 hrs(3-replications); treated or untreated: C. sativus (spot blotch) - time: 48 hrs(3-replications); treated or untreated: water control - time: 12 hrs(3-replications); treated or untreated: water control - time: 24 hrs(3-replications); treated or untreated: water control - time: 36 hrs(3-replications); treated or untreated: water control - time: 48 hrs(3-replications); treated or untreated: P. hordei (leaf rust) - time: 12 hrs(3-replications); treated or untreated: P. hordei (leaf rust) - time: 24 hrs(3-replications); treated or untreated: P. hordei (leaf rust) - time: 36 hrs(3-replications); treated or untreated: P. hordei (leaf rust) - time: 48 hrs(3-replications); treated or untreated: oil control - time: 12 hrs(3-replications); treated or untreated: oil control - time: 24 hrs(3-replications); treated or untreated: oil control - time: 36 hrs(3-replications); treated or untreated: oil control - time: 48 hrs(3-replications)
2011-11-22 | E-GEOD-33401 | biostudies-arrayexpress