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CUT&Tag-seq for H3K27ac in hematopoietic stem cells in bone marrow after 5-FU administration

ABSTRACT: CUT&Tag-seq for H3K27ac in hematopoietic stem cells in bone marrow after 5-FU administration

PROVIDER: PRJNA785987 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17123920.fastq.gz	Fastqsanger.gz
	SRR17123921.fastq.gz	Fastqsanger.gz
	SRR17123922.fastq.gz	Fastqsanger.gz
	SRR17123923.fastq.gz	Fastqsanger.gz
	SRR17123924.fastq.gz	Fastqsanger.gz

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CUT&Tag-seq for H3K27ac in hematopoietic stem cells in bone marrow after 5-FU administration

Project description:Purpose: The goals of this study are to elucidate the underlying mechanism for the regulation of HSC divisions. Methods: Before or after wild type (WT) mice were treated with 5-fluorouracil (5-FU), 12500 cells of HSCs were sorted. These cells were subjected to CUT&Tag-seq to analyze H3K27ac profile by using Next-seq. The sequence reads that passed quality filters were analyzed by Trimmomatic.

2022-01-19 | GSE190148 | GEO

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H3K27ac CUT&Tag iEC reprogramming

Project description:H3K27ac CUT&Tag analysis of Sox17-Erg and Etv2 reprogramming of cardiac fibroblasts into endothelial cells

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CUT&TAG analysis of H3K27ac in granulosa cells from control and POI patients

Project description:To reveal the role of NCOA7 in cellular senescence, we performed the CUT&TAG assay using the H3K27ac antibody to map acetylation in granulosa cells from control and POI patients. We conducted the DNA sequencing of libraries from CUT&TAG assay using the H3K27ac antibody in human primary granulosa cells.

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Role of Setd2 in regulatory T cells [H3K27ac CUT&Tag]

Project description:To determine the molecular regulation of Tregs by Setd2, large intestine Tregs (CD3+CD4+Foxp3-YFP+) from Setd2-deficient and control Tregs were subjected to H3K27ac CUT&Tag analysis.

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Super-enhancer H3K27ac CUT& Tag sequencing of six human gastric cancer tissue samples

Project description:CUT& Tag was performed using H3K27ac antibody to identify the super-enhancers of GC with different growth patterns through ROSE algorithm. Comparison of SE-driven oncogenes in infiltrative and expanding GC was carried out to screen key signatures and candidate genes.

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Genome-wide identification of transcriptional targets for H3K23ac, H3K27ac, and H4K5ac in vascular smooth muscle cells of aneurysmal aortas [CUT&Tag]

Project description:In this study, we found that H3K23ac, H3K27ac, and H4K5ac level are elevated in VSMCs of aneurysmal aortas. To further explore the potential functional significance of H3K23ac, H3K27ac, and H4K5ac in aortic aneurysm, we performed genome-wide cleavage under targets and tagmentation (CUT&Tag) analysis to identify candidate genes regulated by H3K23ac, H3K27ac, or H4K5ac in rat VSMC. Following CUT&Tag, H3K23ac, H3K27ac, or H4K5ac-associated DNAs were amplified using non-biased conditions, labeled, and sequenced with Illumina NovaSeq 150PE.

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H3K9ac and H3K27ac Cut&Tag profiling of wild type and Rbm25-deficient Bone Marrow Derived macrophage

Project description:Purpose: To transcriptome widely reveal histone acetylation modification H3K9ac and H3K27ac regulation by Rbm25, we performed Cleavage Under Targets and Tagmentation (CUT&Tag) analysis of wild-type (WT) and Rbm25-deficiency BMDMs. Methods: Mouse BMDMs were generated from bone marrow cells in RPMI-1640 medium with recombinant mouse M-CSF (20ng/ml). BMDMs were stained to confirm the surface expression of CD11b and F4/80. Cells with purity >97.5% were used for subsequent experiments. WT and Rbm25 deficient BMDMs were stimulated with LPS (100ng/ml) for 4 hours. Results: We carried out CUT&Tag assays using antibody against H3K9ac or H3K27ac, and found the global influence of Rbm25 in regulating H3K9ac and H3K27ac of transcription regulatory regions of key genes for inflammatory macrophage effector function.

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Project description:Cleavave Under Targets and Tagmentation (CUT&TAG) sequencing for YAP, H3K27ac, and H3K27me3 of tongue epithelia-derived, transgene-selected primarily cultured cells from control mice or transgenic mice expressing HPV16E6-E7 and YAP1S127A

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scNanoSeq-CUT&Tag: a long-read single-cell CUT&Tag sequencing method for efficient chromatin modification profiling within individual cells

Project description:We developed scNanoSeq-CUT&Tag, a streamlined method by adapting a modified CUT&Tag protocol to Oxford Nanopore sequencing platform for efficient chromatin modification profiling at single-cell resolution. We firstly tested the performance of scNanoSeq-CUT&Tag on six human cell lines: K562, 293T, GM12878, HG002, H9, HFF1 and adult mouse blood cells, it showed that scNanoSeq-CUT&Tag can accurately distinguish different cell types in vitro and in vivo. Moreover, scNanoSeq-CUT&Tag enables to effectively map the allele-specific epigenomic modifications in the human genome andallows to analyze co-occupancy of histone modifications. Taking advantage of long-read sequencing,scNanoSeq-CUT&Tag can sensitively detect epigenomic state of repetitive elements. In addition, by applying scNanoSeq-CUT&Tag to testicular cells of adult mouse B6D2F1, we demonstrated that scNanoSeq-CUT&Tag maps dynamic epigenetic state changes during mouse spermatogenesis. Finally, we exploited the epigenetic changes of human leukemia cell line K562 during DNA demethylation, it showed that NanoSeq-CUT&Tag can capture H3K27ac signals changes along DNA demethylation. Overall, we prove that scNanoSeq-CUT&Tag is a valuable tool for efficiently probing chromatin state changes within individual cells.

2024-09-06 | GSE249799 | GEO

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