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ABSTRACT: Intestinal microbiota sequencing of perinatal dairy cows

PROVIDER: PRJNA786358 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17184268_1.fastq.gz	Fastqsanger.gz
	SRR17184268_2.fastq.gz	Fastqsanger.gz
	SRR17184269_1.fastq.gz	Fastqsanger.gz
	SRR17184269_2.fastq.gz	Fastqsanger.gz
	SRR17184270_1.fastq.gz	Fastqsanger.gz

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Project description:Intestinal microbiota 16S rRNA gene sequencing of perinatal dairy cows

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A comparative mRNA-seq profiling of the liver, rumen villi, mammalian gland and kidney of Holstein dairy cows with high vs normal milk-urea excretion and with low vs normal (control) crude protein intake

Project description:Nitrogen (N) emissions became a huge topic under environmental and nutrient concerns in dairy farming. Nitrogen is metabolized in cows as a consequence of feed crude protein digestion which is either recycled or excreted via urine, faeces and/or milk. In dairy cows differences between cows in N-recycling and N-emissions have been postulated. This study investigated 24 Holstein dairy cows in late lactation. The experimental design comprises two dietary groups (low (LP) vs normal (NP) crude protein) and two groups of milk urea content, high (HMU) vs low (LMU). Transcriptomic profiles of the liver, rumen, mammalian gland and kidney tissues were comparatively assessed by mRNA sequencing.

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Potassium balance in lactating and non-lactating dairy cows

Project description:M. Berg, J. Plöntzke, S. Leonhard-Marek, K.E. Müller & S. Röblitz. A dynamic model to simulate potassium balance in dairy cows. Journal of Dairy Science 100, 12 (2017). High-performing dairy cows require a particular composition of nutritional ingredients, adapted to their individual requirements and depending on their production status. The optimal dimensioning of minerals in the diet, one being potassium, is indispensable for the prevention of imbalances. Potassium balance in cows is the result of potassium intake, distribution in the organism, and excretion, and it is closely related to glucose and electrolyte metabolism. In this paper, we present a dynamical model for potassium balance in lactating and nonlactating dairy cows based on ordinary differential equations. Parameter values were obtained from clinical trial data and from the literature. To verify the consistency of the model, we present simulation outcomes for 3 different scenarios: potassium balance in (1) nonlactating cows with varying feed intake, (2) nonlactating cows with varying potassium fraction in the diet, and (3) lactating cows with varying milk production levels. The results give insights into the short- and long-term potassium metabolism, providing an important step toward the understanding of the potassium network, the design of prophylactic feed additives, and possible treatment strategies.

2024-09-02 | BIOMD0000000849 | BioModels

The miRNAome of the postpartum dairy cow liver in negative energy balance

Project description:Negative energy balance (NEB) is an altered metabolic state in high yielding cows that occurs during the first few weeks postpartum when energy demands for lactation and maintenance exceed the energy supply from dietary intake. NEB can, in turn, lead to metabolic disorders and to reduced fertility. Alterations in the expression of more than 700 hepatic genes have previously been reported in a study of NEB in postpartum dairy cows. miRNAs (microRNA) are known to mediate many alterations in gene expression post transcriptionally. To study the hepatic miRNA content of postpartum dairy cows, including their overall abundance and differential expression, in mild NEB (MNEB) and severe NEB (SNEB) short read RNA sequencing was carried out.

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Raw single-cell RNA sequencing data of adult rumen tissues

Project description:We performed single-cell RNA-sequencing on the rumen epithelium of dairy cows to construct an epithelial single-cell map of the rumen.

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Project description:Endometrial microbiota in dairy cows

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