Project description:Chromatin organization is a highly orchestrated process that influences gene expression, in part by modulating access of regulatory factors to DNA and nucleosomes. We found that the chromatin accessibility regulator HMGN1, a target of recurrent DNA copy gains in leukemia, controls myeloid differentiation. HMGN1 amplification was associated with increased accessibility, expression, and histone H3K27 acetylation of loci important for hematopoietic stem cell (HSC) function and AML, such as HoxA cluster genes. In vivo, HMGN1 overexpression was linked to decreased quiescence and increased HSC activity in bone marrow transplantation. HMGN1 overexpression also cooperated with the AML-ETO9a fusion oncoprotein to impair myeloid differentiation and enhance leukemia stem cell (LSC) activity. Inhibition of histone acetyltransferases CBP/p300 relieved the HMGN1-associated differentiation block. These data nominate factors that modulate chromatin accessibility as regulators of HSCs and LSCs and suggest that targeting HMGN1 or its downstream effects on histone acetylation could be therapeutically active in AML.

2020-02-25 | PXD017054 | Pride

Chromatin accessibility profiling by ATAC-seq

Project description:Chromatin accessibility profiling by ATAC-seq

| PRJNA780405 | ENA

Profiling chromatin accessibility during stomatal development

Project description:Profiling chromatin accessibility during stomatal development

| PRJNA788402 | ENA

Chromatin accessibility profiling of terminal B cell differentiation [ATAC-Seq]

Project description:Chromatin accessibility profiling of terminal B cell differentiation [ATAC-Seq]

| PRJNA564230 | ENA

Profiling chromatin accessibility of neutrophil subsets in a mouse model of pancreatic cancer [ATAC-Seq]

Project description:Profiling chromatin accessibility of neutrophil subsets in a mouse model of pancreatic cancer [ATAC-Seq]

| PRJNA1023509 | ENA

Multiplex Single Cell Profiling of Chromatin Accessibility by Combinatorial Cellular Indexing [RNA-seq]

Project description:Technical advances have enabled the collection of genome and transcriptome data sets with single-cell resolution. However, single-cell characterization of the epigenome has remained challenging. Furthermore, because cells must be physically separated prior to biochemical processing, conventional single-cell preparatory methods scale linearly. We applied combinatorial cellular indexing to measure chromatin accessibility in thousands of single cells per assay, circumventing the need for compartmentalization of individual cells. We report chromatin accessibility profiles from over 15,000 single cells and use these data to cluster cells on the basis of chromatin accessibility landscapes. We identify modules of coordinately regulated chromatin accessibility at the level of single cells both between and within cell types, with a scalable method that may accelerate progress toward a human cell atlas. 3 replicates from GM12878 and HL-60 cell lines collected for differential gene expression analysis.

2015-05-07 | E-GEOD-68102 | biostudies-arrayexpress

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