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CircRNA microarray for 6 mouse intestinal tissue samples

ABSTRACT: circRNA microarray for 6 mouse intestinal tissue samples

PROVIDER: PRJNA787232 | ENA |

REPOSITORIES: ENA

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circRNA microarray for 6 mouse intestinal tissue samples

Project description:We analyzed the expression profiles of 9,821circRNAs on intestine tissues from mice suffering from sham and intestinal I/R using microarray analysis. And find that 4 circRNAs are up-regulated and 58 are down-regulated significantly.

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Project description:We have completed the Arraystar Human circRNA Array analysis of the 24 samples that you submitted. Total RNA from each sample was quantified using the NanoDrop ND-1000. The sample preparation and microarray hybridization were performed based on the Arraystar’s standard protocols. Briefly, total RNAs were digested with Rnase R (Epicentre, Inc.) to remove linear RNAs and enrich circular RNAs. Then, the enriched circular RNAs were amplified and transcribed into fluorescent cRNA utilizing a random priming method (Arraystar Super RNA Labeling Kit; Arraystar). The labeled cRNAs were hybridized onto the Arraystar Human circRNA Array (8x15K, Arraystar). After having washed the slides, the arrays were scanned by the Agilent Scanner G2505C. Agilent Feature Extraction software (version 11.0.1.1) was used to analyze acquired array images. Quantile normalization and subsequent data processing were performed using the R software package. Differentially expressed circRNAs with statistical significance between two groups were identified through Volcano Plot filtering. Differentially expressed circRNAs between two samples were identified through Fold Change filtering. Hierarchical Clustering was performed to show the distinguishable circRNAs expression pattern among samples.

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