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Phthorimaea operculella

ABSTRACT: Phthorimaea operculella Raw sequence reads

PROVIDER: PRJNA787525 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR17225986_1.fastq.gz	Fastqsanger.gz
	SRR17225986_2.fastq.gz	Fastqsanger.gz
	SRR17225987_1.fastq.gz	Fastqsanger.gz
	SRR17225987_2.fastq.gz	Fastqsanger.gz
	SRR17225988_1.fastq.gz	Fastqsanger.gz

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Project description:In this research, the complete mitochondrial genome (mitogenome) of Phthorimaea operculella was sequenced and annotated. The mitogenome of P. operculella is 15,269 bp in length and contains 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosome RNA (12s and 16srRNA) genes and 1 control region. In addition, we used Endoclita signifier as the outgroup to analyze phylogenetic relationship, and the phylogenetic tree showed the sister relationship between P. operculella and Tuta absoluta.

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Project description:The potato tuberworm, Phthorimaea operculella Zeller, is an oligophagous pest feeding on crops mainly belonging to the family Solanaceae. It is one of the most destructive pests of potato worldwide and attacks foliage and tubers in the field and in storage. However, the lack of a high-quality reference genome has hindered the association of phenotypic traits with their genetic basis. Here, we report on the genome assembly of P. operculella at the chromosomal level. Using Illumina, Nanopore and Hi-C sequencing, a 648.2 Mb genome was generated from 665 contigs, with an N50 length of 3.2 Mb, and 92.0% (596/648.2 Mb) of the assembly was anchored to 29 chromosomes. In total, 16619 genes were annotated, and 92.4% of BUSCO genes were fully represented. The chromosome-level genome of P. operculella will provide a significant resource for understanding the genetic basis for the biological study of this insect, and for promoting the integrative management of this pest in future.

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