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Next Generation Sequencing of Mantle cell lymphoma Granta-519 Transcriptomes

ABSTRACT: Next Generation Sequencing of Mantle cell lymphoma Granta-519 Transcriptomes

PROVIDER: PRJNA787626 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17184343.fastq.gz	Fastqsanger.gz
	SRR17184344.fastq.gz	Fastqsanger.gz
	SRR17184345.fastq.gz	Fastqsanger.gz
	SRR17184346.fastq.gz	Fastqsanger.gz
	SRR17184347.fastq.gz	Fastqsanger.gz

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Next Generation Sequencing of Mantle cell lymphoma Granta-519 Transcriptomes

Project description:Purpose: The goal of this study is to get a deeper insight of whole transcriptome changes and of signaling pathways involved in eliciting the DPN-mediated effects on Granta-519 tumor progression in vivo. Methods: Mantle cell lymphoma cell line Granta-519 cells were grafted to male NSG mice, followed by treated with ESR2 selective agonist DPN or vehicle. When tumor size reached around 1300 mm3, RNA was extracted with RNeasy Plus Mini Kit (Qiagen) for sequencing. Results: ESR2 selective agonist DPN could inhibit cell adhesion, EMT and angiogenesis in vivo which likely involves regulation of VEGFA, FOS, FOSB, CXCR4, MALAT1 and NEAT1. Conclusions: Our study represents the detailed analysis of Granta-519 transcriptomes in vivo experiment after DPN treatment, with biologic replicates, generated by RNA-seq technology.

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