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Transcriptomic analysis of wild-type and amos mutant D. melanogaster third-instar larval antennal discs and adult antennae

ABSTRACT: Transcriptomic analysis of wild-type and amos mutant D. melanogaster third-instar larval antennal discs and adult antennae

PROVIDER: PRJNA787970 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17200239.fastq.gz	Fastqsanger.gz
	SRR17200240.fastq.gz	Fastqsanger.gz
	SRR17200241.fastq.gz	Fastqsanger.gz
	SRR17200242.fastq.gz	Fastqsanger.gz
	SRR17200243.fastq.gz	Fastqsanger.gz

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Transcriptomic analysis of wild-type and amos mutant D. melanogaster third-instar larval antennal discs and adult antennae

Project description:Comparative transcriptomic analysis of Drosophila melanogaster third-instar larval antennal discs and adult antennae from wild-type animals and amos mutant animals.

2021-12-13 | GSE190696 | GEO

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Project description:Transcriptomes of Drosophila melanogaster eye-antennal imaginal discs at three sequential larval stages: late 2nd instar (72h after egg laying (AEL)), mid 3rd instar (96h AEL) and late 3rd instar (120h AEL).

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Gene expression profile of Drosophila third instar larval wing imaginal discs overexpressing netrinA (netA) and frazzled (fra).

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Transcriptome analysis of control (y w) and Paip1 mutant third instar larval wing imaginal discs

Project description:We report the genome-wide RNA profiling of third instar larval wing imaginal discs upon Paip1 mutation

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Analysis of Drosophila melanogaster Third Instar Larval Eye and Antennal Disc Transcriptomes

Project description:The goal of this study was to examine RNA expression levels in the Drosophila larval eye and antennal discs and determine whether higher levels of transcription were correlated with the ability of transgenes to drive pairing with their homologous endogenous loci between chromosomes. Additionally, RNA expression levels were compared between the eye and antennal discs to determine whether increased insulator protein expression contributed to increased pairing in the eye disc.

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Transcriptomic analysis of third instar larval wing imaginal discs of Drosophila melanogaster corto and uL11 mutants

Project description:We report here the transcriptomic analysis of Drosophila melanogaster wing imaginal discs from third instar female larvae mutant for corto (cortoL1/corto420) The reference line was the w1118 genetic background of the mutant lines.

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Gene expression profile of Drosophila third instar larval wing imaginal discs overexpressing netrinA (netA) and frazzled (fra).

Project description:In order to analyze the global changes in gene expression resulting from induction of NetA-Fra signaling, we carried out a microarray experiment comparing Drosophila third instar wing imaginal discs in which Net+Fra had been overexpressed to age matched wild type wing imaginal discs. RNA extracted from both +NetA-Fra overexpression and wildtype third instar imaginal discs were hybridized to the Affymetrix GeneChip Drosophila Genome 2.0 . Heat shock induced GFP-marked clones ectopically expressing NetA+Fra in larvae were generated. Controls for this study included age matched wildtype third instar wing imaginal discs bearing GFP clones which were prepared in the same manner. Total RNA was extracted from dissected +NetA-Fra vs. control third instar wing imaginal discs and hybridized to the Affymetrix GeneChip Drosophila Genome 2.0.

2013-05-21 | E-GEOD-47113 | biostudies-arrayexpress

Expression data from third instar Drosophila eye discs

Project description:Third instar larval eye discs provide an in vivo model for cell cycle exit studies. Posterior to the Second Mitotic Wave proliferation is absent in a wild type eye disc. Inactivating mutations in tumor suppressor-like genes can lead to genome wide changes in gene expression that allow for inappropriate bypass of cell cycle exit signals posterior to the Second Mitotic Wave. In a mosaic tissue comprised of two wild type (Canton) populations (distinguished by presence or absence of GFP) there is roughly a 50/50 distribution of both populations in the tissue. Using the same method but comparing Warts (wts) to wild type you see that Warts mutant tissue is ~70-80% of the eye disc and the rest is wild type tissue. In the comparison of rbf1120a v rbf1120a+wts using the same method for mosaic creation we were able to see a similar ~80-85% of the eye disc as rbf1120a+wts. rbf1120a cells have relatively little advantage over wild type cells, but we were able to use entirely homozygous mutant discs for RNA extraction. We used microarrays to detail the global program of gene expression underlying cell cycle exit and identified distinct classes of up-regulated and down-regulated genes during this process. Drosophila third instar larvea were selected and the eye discs from these animals were prepared for RNA extraction and hybridization on Affymetrix microarrays. We used mosaic larval discs but the majority of the tissue was comprised of the mutant allelic combination we desired due to the proliferative advantage of these mutant cells over wild type cells.

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Genome-wide binding of Cyclin G in Drosophila melanogaster third instar larva wing imaginal discs.

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