Project description:The MDA-MB-468 triplicate-negative breast cancer cells were treated with 18 nmol/L gamabufotalin (CS-6) or DMSO for 24 h. Then, RNA-seq was performed to explore the differentially expressed genes.
Project description:In this experiment, we generated isogenic carboplatin-resistant MDA-MB-468 cells displaying a 5x increase in IC50 in respect to the parental cell line. We set out to compare the transcriptome of these cell lines, together with MDA-MB-468 cells overexpressing a constitutively active form of beta-catenin to understand underlying transcriptional changes supporting stable carboplatin-tolerance.
Project description:To determine the effect ALDH1A3 expression on global gene expression in MDA-MB-231 cells and MDA-MB-468 cells In MDA-MB-231 cells, ALDH1A3 was overexperssed (have low endogenous levels of ALDH1A3) and compared to MSCV empty vector control. In MDA-MB-468 cells that have high endogenous levels of ALDH1A3, ALDH1A3 expresion was reduced with ALDH1A3 shRNA1 and compared to scramble shRNA control.
Project description:A radioresistant MDA-MB-468 cell line, a human TNBC cell lines, was developed by giving a total dose of 50Gy (2gyx5, 4Gyx3, 6Gyx3, and 10Gyx1) to the parental cell line. The trancriptomic changes that occur include enhanced antioxidant response, increase in DNA repair pathways, and invasion/metastasis features.
Project description:To investigate the effects of disrupting Cu homeostasis on MDA-MB-468 cells in vitro, we treated cells with DCAC50, tetrathiomolybdate, or DMSO (control) or sDMEM for 24 hours