Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Alteration in m6A methylome of goat lung under PPRV infection

ABSTRACT: Alteration in m6A methylome of goat lung under PPRV infection

PROVIDER: PRJNA788223 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Alteration in m6A methylome of goat lung under PPRV infection

Project description:We report the application of MeRIP-seq (m6A-specific methylated RNA immunoprecipitation) for m6A site profiling of lung tissue under Peste des petits ruminants virus (PPRV) infection.

2022-12-05 | GSE190718 | GEO

Genome-wide identification and characterization of long non-coding RNAs and mRNAs in goat tissues infected with PPRV

Project description:We have used RNA-Seq to examine long non-coding RNAs (lncRNAs) and mRNAs from PPRV infected goat tissues (Lung, Spleen and Caecum). Our study reveals the prevalence of lncRNAs in goats, and has identified lncRNAs differentially expressed in different tissues of PPRV (Izatnagar94) infected goats, suggesting its important functions during virus infection.

2020-03-17 | GSE130552 | GEO

Transcriptional profiling of different subsets of PBMCs-CD4+, CD8+, CD14+, CD21+, CD335+ reveals differential response to Sungri/96 PPRV vaccination in Goat and Sheep

Project description:We have used RNA-Seq to examine expression profiles of different subsets of PBMCs-CD4+, CD8+, CD14+, CD21+, CD335+ from Goat and Sheep under sungri/96 PPRV vaccination.

2021-02-19 | GSE155504 | GEO

m6A methylome in JQ1 treatment and control in MDA-MB-231 cells.

Project description:We report the application of MeRIP sequencing technology for high-throughput profiling of m6A methylome in breast cancer cells. Comparison of m6A methylome between control and BETi-treated cells revealed the following findings: 1) Significant global alteration of methylation sites due to BETi-treatment (herein defined as BETi-m6A signature). 2) Gene Ontology (GO) analysis of the differential meRIP-seq candidates enriched pathways involved in chromatin modification, RNA splicing and pathways regulating cell fate , reflecting a critical role of m6A in diverse biological processes.

2023-12-31 | GSE219190 | GEO

Expression profile of miRNAs in PBMCs infected with PPRV in goat

Project description:Expression profile of miRNAs in PBMCs infected with PPRV in goat

| PRJNA432057 | ENA

m6A methylome in WT- and K177Q-METTL3 reconstituting MDA-MB-231 cells as well as in METTL3-deficient MDA-MB-231 cells.

Project description:We report the application of MeRIP sequencing technology for high-throughput profiling of m6A methylome in breast cancer cells. Comparison of m6A methylome between METTL3-WT and METTL3-K177Q reconstituting cells revealed the following findings: 1) Significant global alteration of methylation sites due to K177Q mutation (termed as KQ-m6A signature). 2) GO analysis of the differential m6A-MeRIP candidates enriched pathways involved in chromatin modification, RNA splicing, DNA damage, cell cycle, and autophagy, consistent with a critical role of m6A-mediated nuclear biological activities and highlighted generally recognized cellular events associated with tumorigenesis. 3) we dissected potential mechanistic clues explaining the attenuated invasive phenotype in METTL3K177Q reconstituting cells.

2022-09-20 | GSE183014 | GEO

HIV-1 m6A methylome investigation by m6A-SAC-seq

Project description:HIV-1 m6A methylome investigation by m6A-SAC-seq

| PRJNA1142507 | ENA

Goat Peripheral Blood Mononuclear Cells (PBMCs) responses to infection vary with Peste des Petits Ruminants Virus virulence

Project description:Peste des Petits Ruminants (PPR) is a highly infectious disease caused by a virus of the genus Morbillivirus (PPRV), infecting mainly sheep and goat. Susceptibility of host can vary widely with host breed and virus strain. The mechanisms underlying this variability are not well understood. Here, we carried out the first comparative in vitro study on goat peripheral blood mononuclear cells (PBMCs) infected with PPRV strains of different virulence, vaccine strain (N75/1), low- (IC89), and high-virulent strain (MA08). Goat PBMCs were infected by the different strains and stimulated with Concanavalin A (Con A), and proteome changes of these cells were evaluated during the infection. The level of PPRV replication was assessed first by RT-qPCR quantification of viral N mRNA and by labelling the viral N protein inside the cells with specific antibodies and analysed by flow cytometry. The dynamics of PBMC sub-populations were also evaluated by flow cytometry. Our results showed that viral replication is critical for PPRV inhibitory effect on PBMCs proliferation. Highly virulent MA08 strain reached a higher level of replication in ConA-stimulated PBMCs and induced higher mortality compared to other strains. Low-virulent IC89 strain showed the lower replication level and cell proliferative inhibitory capacities. Differences in immune genes expression levels were assessed using RNAseq analysis and protein expression was compared using mass spectrometry. Analysis of these data provided the transcriptional and proteome landscape of PBMCs infected with these strains. The possible association between the transcriptional and proteome landscape and changes in immune cell subpopulations dynamics was explored.

2021-11-03 | PXD024890 | Pride

RNA m6A methylome of Caenorhabditis elegans

Project description:RNA m6A methylome of Caenorhabditis elegans

| PRJNA625719 | ENA

m6A-Label-seq directly reports m6A methylome at base resolution

Project description:m6A-Label-seq directly reports m6A methylome at base resolution

| PRJNA543237 | ENA