Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Analysis of Chinese sheep preimplantation embryo development and block by single-cell DNA methylome sequencing

ABSTRACT: Analysis of Chinese sheep preimplantation embryo development and block by single-cell DNA methylome sequencing

PROVIDER: PRJNA788375 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR17212500_1.fastq.gz	Fastqsanger.gz
	SRR17212500_2.fastq.gz	Fastqsanger.gz
	SRR17212501_1.fastq.gz	Fastqsanger.gz
	SRR17212501_2.fastq.gz	Fastqsanger.gz
	SRR17212502_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 30

Similar Datasets

Analysis of Chinese sheep preimplantation embryo development and block by single-cell DNA methylome sequencing

Project description:The early stages of mammalian embryonic development involve the participation and cooperation of numerous complex processes, including nutritional, genetic, and epigenetic mechanisms. However, in embryos cultured in vitro, a developmental block occurs that affects embryo development and the efficiency of culture. Although the block period is reported to involve the transcriptional repression of maternal genes and transcriptional activation of zygotic genes, how epigenetic factors regulate developmental block is still unclear. In this study, we systematically analyzed whole-genome methylation levels during five stages of sheep oocyte and preimplantation embryo development using SC-WGBS technology. Then, we examined several million CpG sites in individual cells at each evaluated developmental stage to identify the methylation changes that take place during the development of sheep preimplantation embryos. Our results showed that two strong waves of methylation changes occurred, namely, demethylation at the 8- to 16-cell stage and methylation at the 16- to 32-cell stage. Analysis of DNA methylation patterns in different functional regions revealed a stable hypermethylation status in 3'UTRs and gene bodies; however, significant differences were observed in intergenic and promoter regions at different developmental stages. Changes in methylation at different stages of preimplantation embryo development were also compared to investigate the molecular mechanisms involved in sheep embryo development at the methylation level. In conclusion, we report a detailed analysis of the DNA methylation dynamics during the development of sheep preimplantation embryos. Our results provide an explanation for the complex regulatory mechanisms underlying the embryo developmental block based on changes in DNA methylation levels.

2021-12-15 | GSE190746 | GEO

Preimplantation mouse embryo development MOE430B

Project description:Preimplantation mouse embryo development on Affymetrix MOE430B. Zeng et al (2004) Dev Biol 272:483-496. Keywords: other

2004-09-10 | GSE1745 | GEO

Preimplantation mouse embryo development MOE430A

Project description:Preimplantation mouse embryo development study on Affymetrix MOE430A. Zeng et al (2004) Dev Biol 272:483-496. Keywords: other

2004-09-10 | GSE1744 | GEO

Preimplantation mouse embryo development U74Av2

Project description:Preimplantation mouse embryo development study on Affymetrix U74Av2. Zeng et al Dev Biol (2004) 272:483-496. Keywords: other

2004-07-06 | GSE1391 | GEO

Transcription profiling of preimplantation mouse embryo development

Project description:Preimplantation mouse embryo development study on Affymetrix MOE430A. Zeng et al (2004) Dev Biol 272:483-496.

2008-06-11 | E-GEOD-1744 | biostudies-arrayexpress

Isoform-resolved transcriptome of the human preimplantation embryo [PacBio]

Project description:Human preimplantation development is a complex process involving extensive remodeling of gene expression. However, the preimplantation embryo transcriptome has only been annotated using short-read sequencing, which fails to capture full-length mRNAs and associated isoform diversity. We present a novel human embryo transcriptome using integrated long- and short-read RNA sequencing data. Our analysis reveals a total of 110,212 novel isoforms transcribed from known genes containing either a novel combination of known splice sites or at least one novel splice site, and 17,964 isoforms transcribed from completely novel genes located either in antisense direction of known genes or in intergenic space.

2023-09-26 | GSE190547 | GEO

Isoform-resolved transcriptome of the human preimplantation embryo [Illumina]

2023-09-26 | GSE190544 | GEO

Whole-Genome DNA Methylation Dynamics of Sheep Preimplantation Embryo Investigated by Single-Cell DNA Methylome Sequencing.

Project description:The early stages of mammalian embryonic development involve the participation and cooperation of numerous complex processes, including nutritional, genetic, and epigenetic mechanisms. However, in embryos cultured in vitro, a developmental block occurs that affects embryo development and the efficiency of culture. Although the block period is reported to involve the transcriptional repression of maternal genes and transcriptional activation of zygotic genes, how epigenetic factors regulate developmental block is still unclear. In this study, we systematically analyzed whole-genome methylation levels during five stages of sheep oocyte and preimplantation embryo development using single-cell level whole genome bisulphite sequencing (SC-WGBS) technology. Then, we examined several million CpG sites in individual cells at each evaluated developmental stage to identify the methylation changes that take place during the development of sheep preimplantation embryos. Our results showed that two strong waves of methylation changes occurred, namely, demethylation at the 8-cell to 16-cell stage and methylation at the 16-cell to 32-cell stage. Analysis of DNA methylation patterns in different functional regions revealed a stable hypermethylation status in 3'UTRs and gene bodies; however, significant differences were observed in intergenic and promoter regions at different developmental stages. Changes in methylation at different stages of preimplantation embryo development were also compared to investigate the molecular mechanisms involved in sheep embryo development at the methylation level. In conclusion, we report a detailed analysis of the DNA methylation dynamics during the development of sheep preimplantation embryos. Our results provide an explanation for the complex regulatory mechanisms underlying the embryo developmental block based on changes in DNA methylation levels.

| S-EPMC8733409 | biostudies-literature

P300 Regulates Histone Crotonylation and Preimplantation Embryo Development

Project description:P300 Regulates Histone Crotonylation and Preimplantation Embryo Development

| PRJNA1006709 | ENA

Isoform-resolved transcriptome of the human preimplantation embryo

Project description:Isoform-resolved transcriptome of the human preimplantation embryo

| PRJNA787537 | ENA