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ABSTRACT: Bacterial community analysis of frass sample derived from food waste treatment by black soldier fly larvae

PROVIDER: PRJNA788678 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR17224953.fastq.gz	Fastqsanger.gz
	SRR17224954.fastq.gz	Fastqsanger.gz
	SRR17224955.fastq.gz	Fastqsanger.gz
	SRR17224956.fastq.gz	Fastqsanger.gz
	SRR17224957.fastq.gz	Fastqsanger.gz

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Global gene expression analyses of channel catfish after being fed diets with inclusion of frass from black soldier fly larvae

Project description:Frass is the by-product of the larval meal industry and includes larval waste, exoskeleton sheds, and residual feed ingredients. Experimental frass was derived from the larvae of black solder flies (Hermetia illucens) fed distillers dried grains. A 10-week study was conducted to evaluate the effect of dietary levels of frass on the global gene expression of channel catfish, Ictalurus punctatus. Three diets containing 0, 50, and 300 g frass per kg diet were fed to channel catfish (5.24 ± 0.04 g) in quadruplicate aquaria to apparent satiation twice daily. Intestine (n=12 in pools of 3) and liver (n=12 in pools of 3) tissues were taken from fish at the end of the trial and processed for high-throughput Illumina RNA sequencing (RNAseq). Pairwise comparisons identified both up- and down-regulated genes in frass diets compared to no frass controls.

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Dataset from The proteome landscape of the black soldier fly provides insights into protein function and microbial diversity

Project description:The larvae of black soldier fly (BSF) Hermetia illucens (Diptera: Stratiomyidae), has demonstrated ability in the efficient bioconversion of organic waste into a sustainable source of food and feed, but fundamental biology remains to be discovered to exploit their full biodegradative potential. Herein, LC-MS/MS was used to assess the efficiency of eight differing extraction protocols to build foundational knowledge regarding the proteome landscape of both BSF larvae body and gut. No specific protocol was superior in capturing the BSF body and gut proteome, but each yielded complementary information to improve BSF proteome coverage. Protocol-specific functional annotation using protein level information has shown that the selection of extraction buffer can affect protein detection and their associated functional classes within the measured BSF larval gut proteome. Metaproteome analysis on BSF larvae gut has uncovered the prevalence of two bacterial phyla: actinobacteria and proteobacteria. We envisage that comparing a range of extraction protocols and investigating the proteome from the BSF body and gut separately will expand the fundamental knowledge of the BSF proteome and thereby provide translational opportunities for future research to enhance their efficiency for waste degradation and contribution to the circular economy.

2023-03-11 | PXD037977 | Pride

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RNA-seq of stomach and pyloric caeca from Atlantic salmon (Salmo salar) fed diets containing chitin from shrimp and black soldier fly larvae

Project description:Farmed Atlantic salmon was given either a 6 % cellulose diet, a diet containing 6 % shrimp shell chitin or a diet containing 6 % chitin from black soldier fly larvae for a period of 4 weeks. The fish were split into six tanks at the beginning of the experiment; six fish per tank and two tanks per diet. RNA from stomach and pyloric caeca from four fish given each diet was sequenced.

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