Project description:MicroRNAs (miRNAs) and endogenous small interfering RNAs (siRNAs) play an important role in plant stress responses but the small RNA (sRNA) repertoire is poorly characterized in extremphophiles. We report sRNA transcriptomes of leaf and flower tissues of two mangrove species Bruguiera gymnorrhiza and Kandelia candel (Rhizophoraceae) based on Illumina sequencing. A total of 32 known and 5 novel miRNA families were identified collectively.Besides the conserved miR390-TAS3-ARF pathway, B. gymnorrhiza possessed additional high-abundance tasiRNAs derived from the less-conserved regions of TAS3 transcripts with expanded potential targets.his study provides the first comprehensive sRNA transcriptome for mangroves B. gymnorrhiza and K. candel. Comparative analyses revealed that these two species systematically optimize sRNA expression levels, accompanied with rewiring of sRNA regulatory networks, as an evolutionarily strategy for stress adaptations. Furthermore, 24-nt sRNAs mapped to the telomeric repeats (CCCTAAA)n were highly abundant in both B. gymnorrhiza and K. candel.
Project description:The intra sub-species diversity of six strains of Lactococcus lactis subsp. lactis was investigated at the genomic level and in terms of phenotypic and transcriptomic profiles in UF-cheese model. Six strains were isolated from various sources, but all are exhibiting a dairy phenotype. Our results showed that, the six strains exhibited small phenotypic differences since similar behaviour in terms of growth was obtained during cheese ripening while only different acidification capability was detected. Even if all strains displayed high genomic similarities, sharing a high core genome of almost two thousands genes, the expression of this core genome directly in the cheese matrix revealed major strain-specific differences. This strains with the same dairy origin.