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A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis [bam-aramis double mRNAseq]

ABSTRACT: A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis [bam-aramis double mRNAseq]

PROVIDER: PRJNA789193 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17261229.fastq.gz	Fastqsanger.gz
	SRR17261230.fastq.gz	Fastqsanger.gz
	SRR17261231.fastq.gz	Fastqsanger.gz
	SRR17261232.fastq.gz	Fastqsanger.gz

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A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis [bam-aramis double mRNAseq]

Project description:Ribosomal defects perturb stem cell differentiation, causing diseases called ribosomopathies. How ribosome levels control stem cell differentiation is not fully known. Here, we discovered three RNA helicases are required for ribosome biogenesis and for Drosophila oogenesis. Loss of these helicases, which we named Aramis, Athos and Porthos, lead to aberrant stabilization of p53, cell cycle arrest and stalled GSC differentiation. Unexpectedly, Aramis is required for efficient translation of a cohort of mRNAs containing a 5’-Terminal-Oligo-Pyrimidine (TOP)-motif, including mRNAs that encode ribosomal proteins and a conserved p53 inhibitor, Novel Nucleolar protein 1 (Non1). The TOP-motif co-regulates the translation of growth-related mRNAs in mammals. As in mammals, the La-related protein co-regulates the translation of TOP-motif containing RNAs during Drosophila oogenesis. Thus, a previously unappreciated TOP-motif in Drosophila responds to reduced ribosome biogenesis to co-regulate the translation of ribosomal proteins and a p53 repressor, thus coupling ribosome biogenesis to GSC differentiation.

2022-03-24 | GSE190970 | GEO

A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis

Project description:A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis

| PRJNA719154 | ENA

A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis [RNA-seq]

Project description:A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis [RNA-seq]

| PRJNA719155 | ENA

A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis [rRNA_RIP-seq]

Project description:A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis [rRNA_RIP-seq]

| PRJNA719156 | ENA

A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis [Polysome-seq]

Project description:A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis [Polysome-seq]

| PRJNA719159 | ENA

A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis [mRNA_RIP-seq]

Project description:A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis [mRNA_RIP-seq]

| PRJNA719157 | ENA

A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis [aramis mRNA IP]

Project description:A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis [aramis mRNA IP]

| PRJNA789194 | ENA

Transcription profiling of Drosophila ovaries from bam mutant and stwl bam double mutant females

Project description:Abstract: During Drosophila oogenesis, germline stem cell (GSC) identity is maintained largely by preventing the expression of factors that promote differentiation. This is accomplished via the activity of several genes acting either in the GSC or its niche. The translational repressors, Nanos and Pumilio, act in GSCs to prevent differentiation, likely by inhibiting translation of early differentiation factors, while niche signals prevent differentiation by silencing transcription of the differentiation factor Bam. We have found that the DNA-associated protein Stonewall (Stwl) is also required for GSC maintenance. stwl is required cell-autonomously; clones of stwl- germ cells were lost by differentiation, and ectopic Stwl caused an expansion of GSCs. stwl mutants acted as Suppressors of Variegation, indicating stwl normally acts in chromatin-dependent gene repression. In contrast to several previously described GSC maintenance factors, Stwl likely functions epigenetically to prevent GSC differentiation. Stwl-dependent transcriptional repression does not target bam, but rather Stwl represses the expression of many genes, including those that may be targeted by Nanos/Pumilio translational inhibition. Experiment Overall Design: Genetic variation comparison between germ cells of Drosophila ovaries from bam mutant and stwl bam double mutant females. Six total samples were analyzed, with each genotype performed in triplicates.

2008-03-13 | E-GEOD-6928 | biostudies-arrayexpress

SAGA core module subunits regulate gene expression during oogenesis [ChIP-seq]

Project description:SAGA core module subunits regulate gene expression during oogenesis [ChIP-seq]

| PRJNA699611 | ENA

A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis [rRNA_RIP-seq]

2022-03-24 | GSE171347 | GEO